ArticleInfection and immunity1998
Mannose induces the release of cytopathic factors from Acanthamoeba castellanii.
Article in Infection and immunity, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 96 citations in OpenAlex.
- β-1,3-Glucan recognition byApplied and environmental microbiology · 2024Article
- Proteases of Acanthamoeba.Parasitology research · 2023Review
- Biological characteristics and pathogenicity ofFrontiers in microbiology · 2023Review
- Article
- Interaction between Naegleria fowleri and pathogenic Escherichia coli by mannose and changes in N. fowleri protease.Parasitology research · 2022Article
- The biology of Acanthamoeba keratitis.Experimental eye research · 2021Review
- Review
- Article
- Pattern recognition receptors in microbial keratitis.Eye (London, England) · 2015Review
- Superdiffusion dominates intracellular particle motion in the supercrowded cytoplasm of pathogenic Acanthamoeba castellanii.Scientific reports · 2015Article
- Role of phospholipase A₂ (PLA₂) inhibitors in attenuating apoptosis of the corneal epithelial cells and mitigation of Acanthamoeba keratitis.Experimental eye research · 2013Article
- Acanthamoeba-cytopathic protein induces apoptosis and proinflammatory cytokines in human corneal epithelial cells by cPLA2α activation.Investigative ophthalmology & visual science · 2012Article
- Naegleria fowleri lysate induces strong cytopathic effects and pro-inflammatory cytokine release in rat microglial cells.The Korean journal of parasitology · 2011Article
- Phospholipase activities in clinical and environmental isolates of Acanthamoeba.The Korean journal of parasitology · 2011Article
- Amoebicidal activities of alexidine against 3 pathogenic strains of acanthamoeba.Eye & contact lens · 2009Article
- Comparison of specific activity and cytopathic effects of purified 33 kDa serine proteinase from Acanthamoeba strains with different degree of virulence.The Korean journal of parasitology · 2006Article
- Identification and properties of proteases from an Acanthamoeba isolate capable of producing granulomatous encephalitis.BMC microbiology · 2006Article
- Role of contact lens wear, bacterial flora, and mannose-induced pathogenic protease in the pathogenesis of amoebic keratitis.Infection and immunity · 2005Article
- Induction of morphological and electrophysiological changes in hamster cornea after in vitro interaction with trophozoites of Acanthamoeba spp.Infection and immunity · 2004Article
- Characterisation and differentiation of pathogenic and non-pathogenic Acanthamoeba strains by their protein and antigen profiles.Parasitology research · 2004Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Acanthamoeba keratitis is a chronic inflammatory disease of the cornea which is highly resistant to many antimicrobial agents. The pathogenic mechanisms of this disease are poorly understood. However, it is believed that the initial phases in the pathogenesis of Acanthamoeba keratitis involve parasite binding and lysis of the corneal epithelium. These processes were examined in vitro, using Acanthamoeba castellanii trophozoites. Parasites readily adhered to Chinese hamster corneal epithelial cells in vitro; however, parasite binding was strongly inhibited by mannose but not by lactose. Although mannose prevented trophozoite binding, it did not affect cytolysis of corneal epithelial cells. Moreover, mannose treatment induced trophozoites to release cytolytic factors that lysed corneal epithelial cells in vitro. These factors were uniquely induced by mannose because supernatants collected from either untreated trophozoites or trophozoites treated with other sugars failed to lyse corneal cells. The soluble factors were size fractionated in centrifugal concentrators and found to be > or = 100 kDa. Treatment of the supernatants with the serine protease inhibitor phenylmethylsulfonyl fluoride inhibited most, but not all, of the cytopathic activity. These data suggest that the binding of Acanthamoeba to mannosylated proteins on the corneal epithelium may exacerbate the pathogenic cascade by initiating the release of cytolytic factors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.