ArticleJournal of bacteriology1998
Characterization of the dnaG locus in Mycobacterium smegmatis reveals linkage of DNA replication and cell division.
Article in Journal of bacteriology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 33 citations in OpenAlex.
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- Mycobacterium tuberculosis 6C sRNA binds multiple mRNA targets via C-rich loops independent of RNA chaperones.Nucleic acids research · 2019Article
- Directed Evolution of a Mycobacteriophage.Antibiotics (Basel, Switzerland) · 2019Article
- A versatile vector for mycobacterial protein production with a functional minimized acetamidase regulon.Protein science : a publication of the Protein Society · 2017Article
- Rv0004 is a new essential member of the mycobacterial DNA replication machinery.PLoS genetics · 2017Article
- DNA Replication in Mycobacterium tuberculosis.Microbiology spectrum · 2017Review
- Antimycobacterial activity of DNA intercalator inhibitors of Mycobacterium tuberculosis primase DnaG.The Journal of antibiotics · 2015Article
- Systems biology perspectives on minimal and simpler cells.Microbiology and molecular biology reviews : MMBR · 2014Review
- Evaluation of DNA primase DnaG as a potential target for antibiotics.Antimicrobial agents and chemotherapy · 2014Article
- Bacterial growth and cell division: a mycobacterial perspective.Microbiology and molecular biology reviews : MMBR · 2008Review
- Inactivation of the inhA-encoded fatty acid synthase II (FASII) enoyl-acyl carrier protein reductase induces accumulation of the FASI end products and cell lysis of Mycobacterium smegmatis.Journal of bacteriology · 2000Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have isolated a UV-induced temperature-sensitive mutant of Mycobacterium smegmatis that fails to grow at 42 degrees C and exhibits a filamentous phenotype following incubation at the nonpermissive temperature, reminiscent of a defect in cell division. Complementation of this mutant with an M. smegmatis genomic library and subsequent subcloning reveal that the defect lies within the M. smegmatis dnaG gene encoding DNA primase. Sequence analysis of the mutant dnaG allele reveals a substitution of proline for alanine at position 496. Thus, dnaG is an essential gene in M. smegmatis, and DNA replication and cell division are coupled processes in this species. Characterization of the sequences flanking the M. smegmatis dnaG gene shows that it is not part of the highly conserved macromolecular synthesis operon present in other eubacterial species but is part of an operon with a dgt gene encoding dGTPase. The organization of this operon is conserved in Mycobacterium tuberculosis and Mycobacterium leprae, suggesting that regulation of DNA replication, transcription, and translation may be coordinated differently in the mycobacteria than in other bacteria.
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