ArticleNucleic acids research1998
Random-priming in vitro recombination: an effective tool for directed evolution.
Article in Nucleic acids research, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 177 citations in OpenAlex.
- Recent Advances in β-Glucosidase Sequence and Structure Engineering: A Brief Review.Molecules (Basel, Switzerland) · 2023Review
- Discovery of a highly efficient TylF methyltransferase via random mutagenesis for improving tylosin production.Computational and structural biotechnology journal · 2023Article
- From polymerase engineering to semi-synthetic life: artificial expansion of the central dogma.RSC chemical biology · 2022Review
- Innovations in CAZyme gene diversity and its modification for biorefinery applications.Biotechnology reports (Amsterdam, Netherlands) · 2020Review
- Toward fine-tuned metabolic networks in industrial microorganisms.Synthetic and systems biotechnology · 2020Article
- A Continuing Career in Biocatalysis: Frances H. Arnold.ACS catalysis · 2019Article
- In vitro template-change PCR to create single crossover libraries: a case study with B. thuringiensis Cry2A toxins.Scientific reports · 2016Article
- Improving industrial yeast strains: exploiting natural and artificial diversity.FEMS microbiology reviews · 2014Review
- Biotechnology of cold-active proteases.Biology · 2013Article
- Improving low-temperature activity of Sulfolobus acidocaldarius 2-keto-3-deoxygluconate aldolase.Archaea (Vancouver, B.C.) · 2009Article
- Nucleotide exchange and excision technology (NExT) DNA shuffling: a robust method for DNA fragmentation and directed evolution.Nucleic acids research · 2005Article
- Biphenyl dioxygenases: functional versatilities and directed evolution.Journal of bacteriology · 2004Review
- Generating segmental mutations in haloalkane dehalogenase: a novel part in the directed evolution toolbox.Nucleic acids research · 2002Article
- An E. coli expression system for the extracellular secretion of barley alpha-amylase.Journal of protein chemistry · 2001Article
- Understanding thermostability in cytochrome P450 by combinatorial mutagenesis.Protein science : a publication of the Protein Society · 2001Article
- Exchanging the active site between phytases for altering the functional properties of the enzyme.Protein science : a publication of the Protein Society · 2000Article
- An improved PCR-mutagenesis strategy for two-site mutagenesis or sequence swapping between related genes.Nucleic acids research · 1998Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A simple and efficient method for in vitro mutagenesis and recombination of polynucleotide sequences is reported. The method involves priming template polynucleotide(s) with random-sequence primers and extending to generate a pool of short DNA fragments which contain a controllable level of point mutations. The fragments are reassembled during cycles of denaturation, annealing and further enzyme-catalyzed DNA polymerization to produce a library of full-length sequences. Screening or selecting the expressed gene products leads to new variants with improved functions, as demonstrated by the recombination of genes encoding different thermostable subtilisins in order to obtain enzymes more stable than either parent.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.