ArticleNucleic acids research1998
The proximal sequence element (PSE) plays a major role in establishing the RNA polymerase specificity of Drosophila U-snRNA genes.
Article in Nucleic acids research, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 43 citations in OpenAlex.
- The minor spliceosome component U4atac regulates JAK/STAT signaling to modulate hematopoiesis and immune responses in Drosophila melanogaster.Nature communications · 2026Article
- Structural basis of human SNAPc recognizing proximal sequence element of snRNA promoter.Nature communications · 2022Article
- U1 snRNP telescripting: molecular mechanisms and beyond.RNA biology · 2021Review
- An alternative D. melanogaster 7SK snRNP.BMC molecular and cell biology · 2021Article
- Assembly of SNAPc, Bdp1, and TBP on the U6 snRNA Gene Promoter in Drosophila melanogaster.Molecular and cellular biology · 2020Article
- Functional Characterization of Novel U6 RNA Polymerase III Promoters: Their Implication for CRISPR-Cas9-Mediated Gene Editing in Aspergillus oryzae.Current microbiology · 2019Article
- Efficient genome editing of wild strawberry genes, vector development and validation.Plant biotechnology journal · 2018Article
- Transcriptional regulation of snRNAs and its significance for plant development.Journal of plant research · 2017Review
- The Myb domain of the largest subunit of SNAPc adopts different architectural configurations on U1 and U6 snRNA gene promoter sequences.Nucleic acids research · 2014Article
- RIP-seq analysis of eukaryotic Sm proteins identifies three major categories of Sm-containing ribonucleoproteins.Genome biology · 2014Article
- General transcription factors and subunits of RNA polymerase III: Paralogs for promoter- and cell type-specific transcription in multicellular eukaryotes.Transcription · 2010Article
- Identification of SNAPc subunit domains that interact with specific nucleotide positions in the U1 and U6 gene promoters.Molecular and cellular biology · 2010Article
- Computational identification of four spliceosomal snRNAs from the deep-branching eukaryote Giardia intestinalis.PloS one · 2008Article
- Structural RNAs of known and unknown function identified in malaria parasites by comparative genomics and RNA analysis.RNA (New York, N.Y.) · 2007Article
- Silencing of the Pink1 gene expression by conditional RNAi does not induce dopaminergic neuron death in mice.International journal of biological sciences · 2007Article
- Insect small nuclear RNA gene promoters evolve rapidly yet retain conserved features involved in determining promoter activity and RNA polymerase specificity.Nucleic acids research · 2007Article
- The PSEA promoter element of the Drosophila U1 snRNA gene is sufficient to bring DmSNAPc into contact with 20 base pairs of downstream DNA.Nucleic acids research · 2005Article
- Identification of an evolutionarily divergent U11 small nuclear ribonucleoprotein particle in Drosophila.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Architectural arrangement of cloned proximal sequence element-binding protein subunits on Drosophila U1 and U6 snRNA gene promoters.Molecular and cellular biology · 2004Article
- Cloning and characterization of the Drosophila U7 small nuclear RNA.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Most small nuclear RNA (snRNA) genes are transcribed by RNA polymerase II, but some (e.g., U6) are transcribed by RNA polymerase III. In vertebrates a TATA box at a fixed distance downstream of the proximal sequence element (PSE) acts as a dominant determinant for recruiting RNA polymerase III to U6 gene promoters. In contrast, vertebrate snRNA genes that contain a PSE but lack a TATA box are transcribed by RNA polymerase II. In plants, transcription of both classes of snRNA genes requires a TATA box in addition to an upstream sequence element (USE), and polymerase specificity is determined by the spacing between these two core promoter elements. In these examples, the PSE (or USE) is interchangeable between the two classes of snRNA genes. Here we report the surprising finding that the Drosophila U1 and U6 PSEs cannot functionally substitute for each other; rather, determination of RNA polymerase specificity is an intrinsic property of the PSE sequence itself. The alteration of two or three base pairs near the 3'-end of the U1 and U6 PSEs was sufficient to switch the RNA polymerase specificity of Drosophila snRNA promoters in vitro. These findings reveal a novel mechanism for achieving RNA polymerase specificity at insect snRNA promoters.
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