ArticleAntimicrobial agents and chemotherapy1997
Flow cytometric analysis of herpes simplex virus type 1 susceptibility to acyclovir, ganciclovir, and foscarnet.
Article in Antimicrobial agents and chemotherapy, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 41 citations in OpenAlex.
- Immunoinformatic and systems biology approaches to predict and validate peptide vaccines against Epstein-Barr virus (EBV).Scientific reports · 2019Article
- A core extended naphtalene diimide G-quadruplex ligand potently inhibits herpes simplex virus 1 replication.Scientific reports · 2017Article
- Article
- Resistance of herpes simplex viruses to nucleoside analogues: mechanisms, prevalence, and management.Antimicrobial agents and chemotherapy · 2011Review
- The Epstein-Barr virus (EBV)-encoded protein kinase, EBV-PK, but not the thymidine kinase (EBV-TK), is required for ganciclovir and acyclovir inhibition of lytic viral production.Journal of virology · 2010Article
- Surveillance network for herpes simplex virus resistance to antiviral drugs: 3-year follow-up.Journal of clinical microbiology · 2004Article
- Use of a single monoclonal antibody to determine the susceptibilities of herpes simplex virus type 1 and type 2 clinical isolates to acyclovir.Clinical and diagnostic laboratory immunology · 2002Article
- Application of real-time PCR for determination of antiviral drug susceptibility of herpes simplex virus.Antimicrobial agents and chemotherapy · 2002Article
- Comparison between human cytomegalovirus pUL97 and murine cytomegalovirus (MCMV) pM97 expressed by MCMV and vaccinia virus: pM97 does not confer ganciclovir sensitivity.Journal of virology · 2000Article
- Applications of flow cytometry to clinical microbiology.Clinical microbiology reviews · 2000Review
- Rapid ganciclovir susceptibility assay using flow cytometry for human cytomegalovirus clinical isolates.Antimicrobial agents and chemotherapy · 1998Article
- Drug-resistant herpesviruses: should we look for them?European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 1998Article
- Flow cytometric determination of ganciclovir susceptibilities of human cytomegalovirus clinical isolates.Journal of clinical microbiology · 1998Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We established a quantitative flow cytometric method for determination of herpes simplex virus type 1 (HSV-1) susceptibility to acyclovir (ACV), ganciclovir, and foscarnet in vitro. Susceptibility was defined in terms of the drug concentration which reduced the number of cells expressing HSV-1 glycoprotein C (gpC) with a fluorescence intensity of > or =10(2) by 50% (IC50). Flow cytometry allowed us to use a high (1.0) as well as a low (0.005) multiplicity of infection, and determination of the IC50 was possible after one or more viral replicative cycles. IC50s were dependent on virus input and on time postinfection. In mixture experiments, 1 to 2% resistant viruses added to a sensitive strain could be detected. The results obtained by flow cytometry showed a good qualitative correlation with those achieved by cytopathic effect inhibitory assay. However, flow cytometry might detect more quantitative differences in drug susceptibility, especially among resistant strains, as confirmed also by determination of intracellular drug phosphorylation. The mean IC50s for ACV-sensitive strains were 0.45 to 1.47 microM, and those for ACV-resistant strains were between 140 and 3,134 microM. Flow cytometric analysis was fast and accurate, automatizable, and highly reproducible. Flow cytometry may be a more powerful tool than standard cytopathic effect-based assays and could have advantages for the detection of low levels of drug resistance or mixtures of sensitive and resistant virus strains.
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