ArticleProceedings of the National Academy of Sciences of the United States of America1998
A transcription activation system for regulated gene expression in transgenic plants.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 93 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
93 citing papers in PubMed, 214 citations in OpenAlex.
- Dexamethasone-inducible LhGR/pOp system: simple and flexible spatiotemporal control of gene expression.Planta · 2026Review
- A two-step auxin-GA cross-talk regulates organ formation in tomato.Development (Cambridge, England) · 2026Article
- An ABA-ROP toggle switch orchestrates xylem differentiation and cell wall patterning.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Genome wide inherited modifications of the tomato epigenome by trans-activated bacterial CG methyltransferase.Cellular and molecular life sciences : CMLS · 2024Article
- Genomic analysis of a spontaneous unifoliate mutant reveals gene candidates associated with compound leaf development in Vigna unguiculata [L] Walp.Scientific reports · 2024Article
- A PAX6-regulated receptor tyrosine kinase pairs with a pseudokinase to activate immune defense upon oomycete recognition inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- The design of synthetic gene circuits in plants: new components, old challenges.Journal of experimental botany · 2023Review
- Misexpression Approaches for the Manipulation of Flower Development.Methods in molecular biology (Clifton, N.J.) · 2023Article
- CLASS-II KNOX genes coordinate spatial and temporal ripening in tomato.Plant physiology · 2022Article
- Starch biosynthesis in guard cells has features of both autotrophic and heterotrophic tissues.Plant physiology · 2022Article
- MiR172-APETALA2-like genes integrate vernalization and plant age to control flowering time in wheat.PLoS genetics · 2022Article
- The VIL gene CRAWLING ELEPHANT controls maturation and differentiation in tomato via polycomb silencing.PLoS genetics · 2022Article
- Delayed Leaf Senescence by Upregulation of Cytokinin Biosynthesis Specifically in Tomato Roots.Frontiers in plant science · 2022Article
- Optimal BR signalling is required for adequate cell wall orientation in the Arabidopsis root meristem.Development (Cambridge, England) · 2021Article
- Perspectives for epigenetic editing in crops.Transgenic research · 2021Review
- TheGenes · 2021Article
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- Expression ofFrontiers in plant science · 2021Article
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- Regulation of Cell Type-Specific Immunity Networks in Arabidopsis Roots.The Plant cell · 2020Article
33 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A widely applicable promoter system is described that allows a gene of interest to be activated in specific plant tissues after a cross between defined transgenic lines. The promoter, pOp, consists of lac operators cloned upstream of a minimal promoter. No expression was detected from this promoter when placed upstream of a beta-glucuronidase (GUS) reporter gene in transgenic plants. Transcription from the promoter was activated by crossing reporter plants with activator lines that expressed a chimeric transcription factor, LhG4. This factor comprised transcription-activation domain-II from Gal4 of Saccharomyces cerevisiae fused to a mutant lac-repressor that binds its operator with increased affinity. When LhG4 was expressed from the CaMV 35S promoter, the spatial and quantitative expression characteristics of the 35S promoter were exhibited by the GUS reporter. The LhG4/pOp system may be used to study toxic or deleterious gene products, to coordinate the expression of multiple gene products, to restrict transgene phenotypes to the F1 generation, and to generate hybrid seed. The LhG4 system offers spatially regulated gene expression in the tissues of whole plants growing under normal conditions without the need for external intervention. It complements inducible expression systems that offer temporal control of gene expression in tissues that can be treated with inducing chemicals.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.