ArticleProceedings of the National Academy of Sciences of the United States of America1998
Characterization of mammalian translocase of inner mitochondrial membrane (Tim44) isolated from diabetic newborn mouse kidney.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 69 citations in OpenAlex.
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- ALS/FTD mutations in UBQLN2 are linked to mitochondrial dysfunction through loss-of-function in mitochondrial protein import.Human molecular genetics · 2021Article
- Adaptation of a Genetic Screen Reveals an Inhibitor for Mitochondrial Protein Import Component Tim44.The Journal of biological chemistry · 2017Article
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- Higd-1a interacts with Opa1 and is required for the morphological and functional integrity of mitochondria.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Ultrastructural modifications in the mitochondria of hypoxia-adapted Drosophila melanogaster.PloS one · 2012Article
- Epac1-mediated, high glucose-induced renal proximal tubular cells hypertrophy via the Akt/p21 pathway.The American journal of pathology · 2011Article
- Cardiac and skeletal muscle defects in a mouse model of human Barth syndrome.The Journal of biological chemistry · 2011Article
- Discovery of genes related to diabetic nephropathy in various animal models by current techniques.Contributions to nephrology · 2011Article
- Historical chronology of basic and clinical research in diabetic nephropathy and contributions of Japanese scientists.Clinical and experimental nephrology · 2009Review
- Tissue-specific remodeling of the mitochondrial proteome in type 1 diabetic akita mice.Diabetes · 2009Article
- Rap1b GTPase ameliorates glucose-induced mitochondrial dysfunction.Journal of the American Society of Nephrology : JASN · 2008Article
- The role for HNF-1beta-targeted collectrin in maintenance of primary cilia and cell polarity in collecting duct cells.PloS one · 2007Article
- Brucella melitensis triggers time-dependent modulation of apoptosis and down-regulation of mitochondrion-associated gene expression in mouse macrophages.Infection and immunity · 2006Article
- MUC1 oncoprotein is targeted to mitochondria by heregulin-induced activation of c-Src and the molecular chaperone HSP90.Oncogene · 2006Article
- Hyperglycemia: its imminent effects on mammalian nephrogenesis.Pediatric nephrology (Berlin, Germany) · 2005Review
- Identification of adipocyte adhesion molecule (ACAM), a novel CTX gene family, implicated in adipocyte maturation and development of obesity.The Biochemical journal · 2005Article
- Making better transgenic models: conditional, temporal, and spatial approaches.Molecular biotechnology · 2005Review
- Neph1 and nephrin interaction in the slit diaphragm is an important determinant of glomerular permeability.The Journal of clinical investigation · 2003Article
- Identification of a renal-specific oxido-reductase in newborn diabetic mice.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Mammalian translocase of mitochondrial inner membrane (mTim44) was isolated during representational difference analysis of cDNA from diabetic mouse kidney. Streptozotocin-induced diabetic mouse kidney cDNA was prepared and subtracted by normal mouse kidney cDNA. By using one of the isolated cDNA fragments as a screening probe, full-length cDNA of mTim44 was isolated from lambdaZAP kidney cDNA library. At the nucleotide level, mTim44 did not exhibit significant homology with any known genes; however, at the amino acid level, it had 50% similarity and 29% identity with yeast Tim44. C-terminal FLAG epitope-tagged mTim44 fusion protein was transiently expressed in COS7 cells. By using anti-FLAG epitope M2 monoclonal antibody, mTim44 was found to have its subcellular localization associated with mitochondria. By immunoelectron microscopy, mTim44 was seen in the paracrystalline structures within the mitochondria, as well as in their cristae. Mitochondrial import assay of in vitro translated mTim44 indicated that its precursor product ( approximately 50 kDa) was imported and proteolytically processed to a mature approximately 44-kDa protein, and its translocation was inner membrane potential (DeltaPsi)-dependent. Imported mTim44 was protected from protease digestion in which outer membranes were selectively permeabilized with digitonin. The mature form of mTim44 could be recovered in the supernatant of sonicated mitochondrial membrane fraction treated with 0.1 M Na2CO3, pH 11.5. The data indicate that mTim44 is a mitochondrial inner membrane protein, one of the members of the mammalian TIM complex and up-regulated in hyperglycemic states.
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