ArticleMolecular and cellular biology1998
Role of UEV-1, an inactive variant of the E2 ubiquitin-conjugating enzymes, in in vitro differentiation and cell cycle behavior of HT-29-M6 intestinal mucosecretory cells.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers, 1 of them a synthesis that pooled it.
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Who cites it
49 citing papers in PubMed, 1 synthesis or guideline pooled it, 153 citations in OpenAlex.
- Cellular functions and molecular mechanisms of ubiquitination in osteosarcoma.Frontiers in oncology · 2022Pooled it
- Uev1A counteracts oncogeniceLife · 2026Article
- Genetic screening identifies Ube2v1 as a suppressor of immunoglobulin class switch recombination in CH12F3 cells.Molecular biology reports · 2025Article
- Identification of membrane curvature sensing motifs essential for VPS37A phagophore recruitment and autophagosome closure.Communications biology · 2024Article
- Article
- Diversity, Origin and Evolution of the ESCRT Systems.bioRxiv : the preprint server for biology · 2024Article
- STING signalling is terminated through ESCRT-dependent microautophagy of vesicles originating from recycling endosomes.Nature cell biology · 2023Article
- Aging and sperm signals alter DNA break formation and repair in the C. elegans germline.PLoS genetics · 2022Article
- Asgard archaea shed light on the evolutionary origins of the eukaryotic ubiquitin-ESCRT machinery.Nature communications · 2022Article
- Uev1A promotes breast cancer cell migration by up-regulating CT45A expression via the AKT pathway.BMC cancer · 2021Article
- The Molecular Basis of Ubiquitin-Conjugating Enzymes (E2s) as a Potential Target for Cancer Therapy.International journal of molecular sciences · 2021Review
- Linc00426 accelerates lung adenocarcinoma progression by regulating miR-455-5p as a molecular sponge.Cell death & disease · 2020Article
- Leishmania differentiation requires ubiquitin conjugation mediated by a UBC2-UEV1 E2 complex.PLoS pathogens · 2020Article
- The Ubiquitin Conjugating Enzyme: An Important Ubiquitin Transfer Platform in Ubiquitin-Proteasome System.International journal of molecular sciences · 2020Review
- Genetic susceptibility may modify the association between cell phone use and thyroid cancer: A population-based case-control study in Connecticut.Environmental research · 2020Article
- Non-cell autonomous mechanism of Parkinson's disease pathology caused by G2019S LRRK2 mutation in Ashkenazi Jewish patient: Single cell analysis.Brain research · 2019Review
- Transcriptomic Analysis ofFrontiers in psychiatry · 2019Article
- Uev1A promotes breast cancer cell survival and chemoresistance through the AKT-FOXO1-BIM pathway.Cancer cell international · 2019Article
- Uev1A-Ubc13 promotes colorectal cancer metastasis through regulatingOncotarget · 2018Article
- Uev1A facilitates osteosarcoma differentiation by promoting Smurf1-mediated Smad1 ubiquitination and degradation.Cell death & disease · 2017Article
Corrections and comments
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Authors and funding
16 authors at 7 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
By means of differential RNA display, we have isolated a cDNA corresponding to transcripts that are down-regulated upon differentiation of the goblet cell-like HT-29-M6 human colon carcinoma cell line. These transcripts encode proteins originally identified as CROC-1 on the basis of their capacity to activate transcription of c-fos. We show that these proteins are similar in sequence, and in predicted secondary and tertiary structure, to the ubiquitin-conjugating enzymes, also known as E2. Despite the similarities, these proteins lack a critical cysteine residue essential for the catalytic activity of E2 enzymes and, in vitro, they do not conjugate or transfer ubiquitin to protein substrates. These proteins constitute a distinct subfamily within the E2 protein family and are highly conserved in phylogeny from yeasts to mammals. Therefore, we have designated them UEV (ubiquitin-conjugating E2 enzyme variant) proteins, defined as proteins similar in sequence and structure to the E2 ubiquitin-conjugating enzymes but lacking their enzymatic activity (HW/GDB-approved gene symbol, UBE2V). At least two human genes code for UEV proteins, and one of them, located on chromosome 20q13.2, is expressed as at least four isoforms, generated by alternative splicing. All human cell types analyzed expressed at least one of these isoforms. Constitutive expression of exogenous human UEV in HT-29-M6 cells inhibited their capacity to differentiate upon confluence and caused both the entry of a larger proportion of cells in the division cycle and an accumulation in G2-M. This was accompanied with a profound inhibition of the mitotic kinase, cdk1. These results suggest that UEV proteins are involved in the control of differentiation and could exert their effects by altering cell cycle distribution.
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