ArticleMolecular and cellular biology1998
Involvement of negative cofactor NC2 in active repression by zinc finger-homeodomain transcription factor AREB6.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 54 citations in OpenAlex.
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- Intrinsic Balance between ZEB Family Members Is Important for Melanocyte Homeostasis and Melanoma Progression.Cancers · 2020Review
- Transcription elements AREB6 and miR-34a affect apoptosis of PAMs by regulating the expression of SS2-related gene PPP1R11.Cell cycle (Georgetown, Tex.) · 2019Article
- Analysis of SLC4A11, ZEB1, LOXHD1, COL8A2 and TCF4 gene sequences in a multi-generational family with late-onset Fuchs corneal dystrophy.International journal of molecular medicine · 2016Article
- Evolutionary functional analysis and molecular regulation of the ZEB transcription factors.Cellular and molecular life sciences : CMLS · 2012Review
- Genome-wide association study for atopy and allergic rhinitis in a Singapore Chinese population.PloS one · 2011Article
- Expanding roles of ZEB factors in tumorigenesis and tumor progression.American journal of cancer research · 2011Article
- Plant NAC-type transcription factor proteins contain a NARD domain for repression of transcriptional activation.Planta · 2010Article
- Novel mutations in the ZEB1 gene identified in Czech and British patients with posterior polymorphous corneal dystrophy.Human mutation · 2007Article
- Rotavirus infection alters peripheral T-cell homeostasis in children with acute diarrhea.Journal of virology · 2007Article
- Structure and expression of mobile ETnII retroelements and their coding-competent MusD relatives in the mouse.Journal of virology · 2003Article
- Multi-protein complexes in eukaryotic gene transcription.Plant molecular biology · 2002Review
- Molecular interaction and synergistic activation of a promoter by Six, Eya, and Dach proteins mediated through CREB binding protein.Molecular and cellular biology · 2002Article
- Cell-specific phosphorylation of Zfhep transcription factor.Biochemical and biophysical research communications · 2002Article
- Yeast NC2 associates with the RNA polymerase II preinitiation complex and selectively affects transcription in vivo.Molecular and cellular biology · 2001Article
- Isolation of a novel family of C(2)H(2) zinc finger proteins implicated in transcriptional repression mediated by chicken ovalbumin upstream promoter transcription factor (COUP-TF) orphan nuclear receptors.The Journal of biological chemistry · 2000Article
- A single point mutation in TFIIA suppresses NC2 requirement in vivo.The EMBO journal · 2000Article
- Alleviation of human papillomavirus E2-mediated transcriptional repression via formation of a TATA binding protein (or TFIID)-TFIIB-RNA polymerase II-TFIIF preinitiation complex.Molecular and cellular biology · 2000Article
- Identification of CtBP1 and CtBP2 as corepressors of zinc finger-homeodomain factor deltaEF1.Molecular and cellular biology · 1999Article
- p53-mediated repression of alpha-fetoprotein gene expression by specific DNA binding.Molecular and cellular biology · 1999Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The transcription factor AREB6 contains a homeodomain flanked by two clusters of Krüppel type C2H2 zinc fingers. AREB6 binds to the E-box consensus sequence, CACCTGT, through either the N- or the C-terminal zinc finger cluster. To gain insights into the molecular mechanism by which AREB6 activates and represses gene expression, we analyzed the domain structure of AREB6 in the context of a heterologous DNA-binding domain by transient-transfection assays. The C-terminal region spanning amino acids 1011 to 1124 was identified as a conventional acidic activation domain. The region containing amino acids 754 to 901, which was identified as a repression domain, consists of 40% hydrophobic amino acids displaying no sequence similarities to other known repression domains. This region repressed transcription in vitro in a HeLa nuclear extract but not in reconstituted transcription systems consisting of transcription factor IID (TFIID), TFIIB, TFIIE, TFIIH/F, and RNA polymerase II. The addition of recombinant negative cofactor NC2 (NC2alpha/DRAP1 and NC2beta/Dr1) to the reconstituted transcription system restored the activity of the AREB6 repression domain. We further demonstrated interactions between the AREB6 repression domain and NC2alpha in yeast two-hybrid assay. Our findings suggest a mechanism of transcriptional repression that is mediated by the general cofactor NC2.
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