ArticleThe Journal of general physiology1998
Properties of an inwardly rectifying ATP-sensitive K+ channel in the basolateral membrane of renal proximal tubule.
Article in The Journal of general physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 37 citations in OpenAlex.
- Epithelial transport inThe Journal of general physiology · 2017Review
- Molecular aspects of structure, gating, and physiology of pH-sensitive background K2P and Kir K+-transport channels.Physiological reviews · 2015Review
- Basolateral membrane K+ channels in renal epithelial cells.American journal of physiology. Renal physiology · 2012Review
- Interaction between Calcineurin and Ca/Calmodulin Kinase-II in Modulating Cellular Functions.Enzyme research · 2011Article
- Role of calcineurin-mediated dephosphorylation in modulation of an inwardly rectifying K+ channel in human proximal tubule cells.The Journal of membrane biology · 2009Article
- The effect of shear stress on the basolateral membrane potential of proximal convoluted tubule of the rat kidney.Pflugers Archiv : European journal of physiology · 2007Article
- Phosphorylation regulates an inwardly rectifying ATP-sensitive K(+)- conductance in proximal tubule cells of frog kidney.The Journal of membrane biology · 2005Article
- Glibenclamide depletes ATP in renal proximal tubular cells by interfering with mitochondrial metabolism.British journal of pharmacology · 2005Article
- Molecular diversity and regulation of renal potassium channels.Physiological reviews · 2005Review
- An inward rectifier K(+) channel at the basolateral membrane of the mouse distal convoluted tubule: similarities with Kir4-Kir5.1 heteromeric channels.The Journal of physiology · 2002Article
- Sulphonylurea drugs reduce hypoxic damage in the isolated perfused rat kidney.British journal of pharmacology · 2000Article
- Volume regulatory responses of basolateral membrane vesicles from Necturus enterocytes: role of the cytoskeleton.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Effects of intra- and extracellular acidifications on single channel Kir2.3 currents.The Journal of physiology · 1999Article
- Expression of a functional Kir4 family inward rectifier K+ channel from a gene cloned from mouse liver.The Journal of physiology · 1999Article
- Regulation of an inwardly rectifying ATP-sensitive K+ channel in the basolateral membrane of renal proximal tubule.The Journal of general physiology · 1998Article
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Authors and funding
3 authors at 2 institutions in 1 country.
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Abstract
The potassium conductance of the basolateral membrane (BLM) of proximal tubule cells is a critical regulator of transport since it is the major determinant of the negative cell membrane potential and is necessary for pump-leak coupling to the Na+,K+-ATPase pump. Despite this pivotal physiological role, the properties of this conductance have been incompletely characterized, in part due to difficulty gaining access to the BLM. We have investigated the properties of this BLM K+ conductance in dissociated, polarized Ambystoma proximal tubule cells. Nearly all seals made on Ambystoma cells contained inward rectifier K+ channels (gammaslope, in = 24.5 +/- 0.6 pS, gammachord, out = 3.7 +/- 0.4 pS). The rectification is mediated in part by internal Mg2+. The open probability of the channel increases modestly with hyperpolarization. The inward conducting properties are described by a saturating binding-unbinding model. The channel conducts Tl+ and K+, but there is no significant conductance for Na+, Rb+, Cs+, Li+, NH4+, or Cl-. The channel is inhibited by barium and the sulfonylurea agent glibenclamide, but not by tetraethylammonium. Channel rundown typically occurs in the absence of ATP, but cytosolic addition of 0. 2 mM ATP (or any hydrolyzable nucleoside triphosphate) sustains channel activity indefinitely. Phosphorylation processes alone fail to sustain channel activity. Higher doses of ATP (or other nucleoside triphosphates) reversibly inhibit the channel. The K+ channel opener diazoxide opens the channel in the presence of 0.2 mM ATP, but does not alleviate the inhibition of millimolar doses of ATP. We conclude that this K+ channel is the major ATP-sensitive basolateral K+ conductance in the proximal tubule.
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