ArticleThe Journal of general physiology1998
Protease modulation of the activity of the epithelial sodium channel expressed in Xenopus oocytes.
Article in The Journal of general physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed, 183 citations in OpenAlex.
- Structural insights into subunit-dependent functional regulation in epithelial sodium channels.Structure (London, England : 1993) · 2025Article
- Nephrotic Syndrome: From Pathophysiology to Novel Therapeutic Approaches.Biomedicines · 2024Review
- Elevated intracellular NaProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Transmembrane serine protease 2 (TMPRSS2) proteolytically activates the epithelial sodium channel (ENaC) by cleaving the channel's γ-subunit.The Journal of biological chemistry · 2022Article
- Aldosterone-Regulated Sodium Transport and Blood Pressure.Frontiers in physiology · 2022Review
- Osmotic tolerance of avian erythrocytes to complete hemolysis in solute free water.Scientific reports · 2019Article
- Epithelial NaAnnual review of physiology · 2018Review
- Protease-activated receptors in kidney disease progression.American journal of physiology. Renal physiology · 2016Review
- The function and regulation of acid-sensing ion channels (ASICs) and the epithelial Na(+) channel (ENaC): IUPHAR Review 19.British journal of pharmacology · 2016Review
- Role of Epithelium Sodium Channel in Bone Formation.Chinese medical journal · 2016Review
- Sodium retention and volume expansion in nephrotic syndrome: implications for hypertension.Advances in chronic kidney disease · 2015Review
- Sensitisation of TRPV4 by PAR2 is independent of intracellular calcium signalling and can be mediated by the biased agonist neutrophil elastase.Pflugers Archiv : European journal of physiology · 2015Article
- Interacting domains in the epithelial sodium channel that mediate proteolytic activation.Channels (Austin, Tex.) · 2015Article
- A long isoform of the epithelial sodium channel alpha subunit forms a highly active channel.Channels (Austin, Tex.) · 2015Article
- Demonstration of proteolytic activation of the epithelial sodium channel (ENaC) by combining current measurements with detection of cleavage fragments.Journal of visualized experiments : JoVE · 2014Article
- Functional analysis of a missense mutation in the serine protease inhibitor SPINT2 associated with congenital sodium diarrhea.PloS one · 2014Article
- Mechanism of action of novel lung edema therapeutic AP301 by activation of the epithelial sodium channel.Molecular pharmacology · 2013Article
- ENaC regulation by proteases and shear stress.Current molecular pharmacology · 2013Review
- Acid-sensing ion channels in pathological conditions.Advances in experimental medicine and biology · 2013Review
- Plasmin and chymotrypsin have distinct preferences for channel activating cleavage sites in the γ subunit of the human epithelial sodium channel.The Journal of general physiology · 2012Article
12 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have investigated the effect of extracellular proteases on the amiloride-sensitive Na+ current (INa) in Xenopus oocytes expressing the three subunits alpha, beta, and gamma of the rat or Xenopus epithelial Na+ channel (ENaC). Low concentrations of trypsin (2 microg/ml) induced a large increase of INa within a few minutes, an effect that was fully prevented by soybean trypsin inhibitor, but not by amiloride. A similar effect was observed with chymotrypsin, but not with kallikrein. The trypsin-induced increase of INa was observed with Xenopus and rat ENaC, and was very large (approximately 20-fold) with the channel obtained by coexpression of the alpha subunit of Xenopus ENaC with the beta and gamma subunits of rat ENaC. The effect of trypsin was selective for ENaC, as shown by the absence of effect on the current due to expression of the K+ channel ROMK2. The effect of trypsin was not prevented by intracellular injection of EGTA nor by pretreatment with GTP-gammaS, suggesting that this effect was not mediated by G proteins. Measurement of the channel protein expression at the oocyte surface by antibody binding to a FLAG epitope showed that the effect of trypsin was not accompanied by an increase in the channel protein density, indicating that proteolysis modified the activity of the channel present at the oocyte surface rather than the cell surface expression. At the single channel level, in the cell-attached mode, more active channels were observed in the patch when trypsin was present in the pipette, while no change in channel activity could be detected when trypsin was added to the bath solution around the patch pipette. We conclude that extracellular proteases are able to increase the open probability of the epithelial sodium channel by an effect that does not occur through activation of a G protein-coupled receptor, but rather through proteolysis of a protein that is either a constitutive part of the channel itself or closely associated with it.
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