ArticleProtein science : a publication of the Protein Society1997
Crystallization and preliminary X-ray studies of I-PpoI: a nuclear, intron-encoded homing endonuclease from Physarum polycephalum.
Article in Protein science : a publication of the Protein Society, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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8 citing papers in PubMed, 15 citations in OpenAlex.
- A synthetic sex ratio distortion system for the control of the human malaria mosquito.Nature communications · 2014Article
- Article
- A new method to efficiently induce a site-specific double-strand break in the fission yeast Schizosaccharomyces pombe.Yeast (Chichester, England) · 2012Article
- Homing endonucleases: from basics to therapeutic applications.Cellular and molecular life sciences : CMLS · 2010Review
- Crystallization and preliminary X-ray diffraction analysis on the homing endonuclease I-Dmo-I in complex with its target DNA.Acta crystallographica. Section F, Structural biology and crystallization communications · 2007Article
- The C-terminal loop of the homing endonuclease I-CreI is essential for site recognition, DNA binding and cleavage.Nucleic acids research · 2007Article
- Thermodynamics of DNA target site recognition by homing endonucleases.Nucleic acids research · 2007Article
- Altered target site specificity variants of the I-PpoI His-Cys box homing endonuclease.Nucleic acids research · 2007Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The homing endonuclease I-PpoI is encoded by an optional third intron, Pp LSU 3, found in nuclear, extrachromosomal copies of the Physarum polycephalum 26S rRNA gene. This endonuclease promotes the lateral transfer or "homing" of its encoding intron by recognizing and cleaving a partially symmetric, 15 bp homing site in 26S rDNA alleles that lack the Pp LSU 3 intron. The open reading frame encoding I-PpoI has been subcloned, and the endonuclease has been overproduced in E. coli. Purified recombinant I-PpoI has been co-crystallized with a 21 bp homing site DNA duplex. The crystals belong to space group P3(1)21, with unit cell dimensions a = b = 114 A, c = 89 A. The results of initial X-ray diffraction experiments indicate that the asymmetric unit contains an enzyme homodimer and one duplex DNA molecule, and that the unit cell has a specific volume of 3.4 A3/dalton. These experiments also provide strong evidence that I-PpoI contains several bound zinc ions as part of its structure.
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