ArticlePlant physiology1997
Biochemical and molecular biological characterization of CAC2, the Arabidopsis thaliana gene coding for the biotin carboxylase subunit of the plastidic acetyl-coenzyme A carboxylase.
Article in Plant physiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 43 citations in OpenAlex.
- Article
- 3D genome structural variations play important roles in regulating seed oil content of Brassica napus.Plant communications · 2024Article
- Snapshot of four mature quinoa (Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- Chloroplast Lipids Metabolism and Function. A Redox Perspective.Frontiers in plant science · 2021Review
- Non-Catalytic Subunits Facilitate Quaternary Organization of Plastidic Acetyl-CoA Carboxylase.Plant physiology · 2020Article
- Profiling protein kinases and other ATP binding proteins in Arabidopsis using Acyl-ATP probes.Molecular & cellular proteomics : MCP · 2013Article
- Reverse-genetic analysis of the two biotin-containing subunit genes of the heteromeric acetyl-coenzyme A carboxylase in Arabidopsis indicates a unidirectional functional redundancy.Plant physiology · 2011Article
- Megadalton complexes in the chloroplast stroma of Arabidopsis thaliana characterized by size exclusion chromatography, mass spectrometry, and hierarchical clustering.Molecular & cellular proteomics : MCP · 2010Article
- A tale of two functions: enzymatic activity and translational repression by carboxyltransferase.Nucleic acids research · 2010Article
- Storage reserve accumulation in Arabidopsis: metabolic and developmental control of seed filling.The arabidopsis book · 2008Article
- Article
- Disruption of plE2, the gene for the E2 subunit of the plastid pyruvate dehydrogenase complex, in Arabidopsis causes an early embryo lethal phenotype.Plant molecular biology · 2003Article
- Brassicaceae express multiple isoforms of biotin carboxyl carrier protein in a tissue-specific manner.Plant physiology · 2001Article
- Coordinate regulation of the nuclear and plastidic genes coding for the subunits of the heteromeric acetyl-coenzyme A carboxylase.Plant physiology · 2000Article
- Light-dependent changes in redox status of the plastidic acetyl-CoA carboxylase and its regulatory component.The Biochemical journal · 1999Article
- A multisubunit acetyl coenzyme A carboxylase from soybean.Plant physiology · 1999Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The biotin carboxylase subunit of the heteromeric chloroplastic acetyl-coenzyme A carboxylase (ACCase) of Arabidopsis thaliana is coded by a single gene (CAC2), which is interrupted by 15 introns. The cDNA encodes a deduced protein of 537 amino acids with an apparent N-terminal chloroplast-targeting transit peptide. Antibodies generated to a glutathione S-transferase-CAC2 fusion protein react solely with a 51-kD polypeptide of Arabidopsis; these antibodies also inhibit ACCase activity in extracts of Arabidopsis. The entire CAC2 cDNA sequence was expressed in Escherichia coli and the resulting recombinant biotin carboxylase was enzymatically active in carboxylating free biotin. The catalytic properties of the recombinant biotin carboxylase indicate that the activity of the heteromeric ACCase may be regulated by light-/dark-induced changes in stromal pH. The CAC2 gene is maximally expressed in organs and tissues that are actively synthesizing fatty acids for membrane lipids or oil deposition. The observed expression pattern of CAC2 mirrors that previously reported for the CAC1 gene (J.-K. Choi, F. Yu, E.S. Wurtele, B.J. Nikolau [1995] Plant Physiol 109: 619-625; J. Ke, J.-K. Choi, M. Smith, H.T. Horner, B.J. Nikolau, E.S. Wurtele [1997] Plant Physiol 113: 357-365), which codes for the biotin carboxyl carrier subunit of the heteromeric ACCase. This coordination is probably partially established by coordinate transcription of the two genes. This hypothesis is consistent with the finding that the CAC2 and CAC1 gene promoters share a common set of sequence motifs that may be important in guiding the transcription of these genes.
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