ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1998
Amphiphysin I antisense oligonucleotides inhibit neurite outgrowth in cultured hippocampal neurons.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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Who cites it
32 citing papers in PubMed, 101 citations in OpenAlex.
- Association of cerebrospinal fluid amphiphysin-1 levels with cognition and Alzheimer's disease pathology biomarkers.Frontiers in aging neuroscience · 2026Article
- AMPH-1 As A Critical Tumor Suppressor That Inhibits Osteosarcoma Progression.Cancer management and research · 2019Article
- AMPH-1 is critical for breast cancer progression.Journal of Cancer · 2018Article
- Network-based proteomic approaches reveal the neurodegenerative, neuroprotective and pain-related mechanisms involved after retrograde axonal damage.Scientific reports · 2015Article
- Dynamin1 is a novel target for IRSp53 protein and works with mammalian enabled (Mena) protein and Eps8 to regulate filopodial dynamics.The Journal of biological chemistry · 2014Article
- Steering neuronal growth cones by shifting the imbalance between exocytosis and endocytosis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Amphiphysin I but not dynamin I nor synaptojanin mRNA expression increased after repeated methamphetamine administration in the rat cerebrum and cerebellum.Journal of neural transmission (Vienna, Austria : 1996) · 2013Article
- Charcot-Marie-Tooth disease and intracellular traffic.Progress in neurobiology · 2012Review
- Single vesicle imaging indicates distinct modes of rapid membrane retrieval during nerve growth.BMC biology · 2012Article
- Lack of the endosomal SNAREs vti1a and vti1b led to significant impairments in neuronal development.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Dynamic interaction of amphiphysin with N-WASP regulates actin assembly.The Journal of biological chemistry · 2009Article
- Identification of a developmentally regulated pathway of membrane retrieval in neuronal growth cones.Journal of cell science · 2008Article
- Astrocytic Ca(2+) waves guide CNS growth cones to remote regions of neuronal activity.PloS one · 2008Article
- Clathrin-mediated endocytosis: the physiological mechanism of vesicle retrieval at hippocampal synapses.The Journal of physiology · 2007Review
- Amphiphysin 1 is important for actin polymerization during phagocytosis.Molecular biology of the cell · 2007Article
- The BAR domain proteins: molding membranes in fission, fusion, and phagy.Microbiology and molecular biology reviews : MMBR · 2006Review
- SNX9 regulates dynamin assembly and is required for efficient clathrin-mediated endocytosis.Molecular biology of the cell · 2005Article
- Impaired synaptic plasticity and learning in mice lacking beta-adducin, an actin-regulating protein.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Regulated delivery of AMPA receptor subunits to the presynaptic membrane.The EMBO journal · 2003Article
- The use of synthetic polymers for delivery of therapeutic antisense oligodeoxynucleotides.Biomaterials · 2002Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Amphiphysin I is an SH3 domain-containing neuronal protein, enriched in axon terminals, which was reported to act as a physiological binding partner for dynamin I in synaptic vesicle endocytosis. Rvs167 and Rvs161, the yeast homologs of amphiphysin I, have been implicated in endocytosis, actin function, and cell polarity. Now we have explored the possibility that amphiphysin I also may have a role in actin dynamics and cell polarity by testing the effect of amphiphysin I suppression on neurite outgrowth. Freshly plated hippocampal neurons were exposed to antisense oligonucleotides via a new delivery system based on a polycationic amphipathic polymer, PS980. Western blot analysis revealed that amphiphysin I levels steadily increased with neuronal differentiation, whereas in antisense-treated cultures amphiphysin I levels were reduced to approximately 10% of control levels at 48 hr. Concomitantly, a collapse of growth cones and a severe inhibition of neurite outgrowth and axon formation were observed. A similar effect was observed previously after dynamin I suppression in the same culture system (). We also have found that amphiphysin I and dynamin I colocalize in developing neurons at all developmental stages and that a pool of both proteins is colocalized with actin patches at the leading edge of growth cones. Our findings suggest a conserved role of the amphiphysin protein family in the dynamics of the cortical cell cytoskeleton and provide new evidence for a close functional link between amphiphysin I and dynamin I.
Indexed as
Identifiers
9412489PMC6793426W2151766851What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.