ArticleThe Journal of cell biology1997
A novel family of serine/threonine kinases participating in spermiogenesis.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
38 citing papers in PubMed, 96 citations in OpenAlex.
- Article
- Article
- The late chromatoid body component TSSK2 is involved in translational regulation in elongating spermatids in mice.Reproduction (Cambridge, England) · 2025Article
- Testis-specific serine/threonine kinase 3 regulates the size of sperm reservoir in Anopheles stephensi.Molecular genetics and genomics : MGG · 2025Article
- Identification of TSSK1 and TSSK2 as Novel Targets for Male Contraception.Biomolecules · 2025Article
- Identification of YBX2 and TSKS As STK33 Interacting Proteins in Testicular Germ Cells.Reproductive sciences (Thousand Oaks, Calif.) · 2025Article
- The LKB1-TSSK1B axis controls YAP phosphorylation to regulate the Hippo-YAP pathway.Cell death & disease · 2024Article
- Expression of Testis-specific Serine/Threonine Kinases during the Reproductive and Nonreproductive Seasons and Their Localization in Mature Spermatozoa of Tree Shrews (Comparative medicine · 2023Article
- Broad phosphorylation mediated by testis-specific serine/threonine kinases contributes to spermiogenesis and male fertility.Nature communications · 2023Article
- TSKS localizes to nuage in spermatids and regulates cytoplasmic elimination during spermiation.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- The Biological Characteristics and Differential Expression Patterns ofAnimals : an open access journal from MDPI · 2023Article
- Using the Culex pipiens sperm proteome to identify elements essential for mosquito reproduction.PloS one · 2023Article
- Biogenesis ofScience advances · 2022Article
- Differential Distribution and Activity Profile of Acylpeptide Hydrolase in the Rat Seminiferous Epithelium.Biomedicines · 2022Article
- Role of testis‑specific serine kinase 1B in undiagnosed male infertility.Molecular medicine reports · 2022Article
- Identification of the TSSK4 Alternative Spliceosomes and Analysis of the Function of the TSSK4 Protein in Yak (Animals : an open access journal from MDPI · 2022Article
- TSSK3, a novel target for male contraception, is required for spermiogenesis.Molecular reproduction and development · 2021Article
- Testis-specific serine kinase protein family in male fertility and as targets for non-hormonal male contraception†.Biology of reproduction · 2020Review
- Transcriptome profiling of developing testes and spermatogenesis in the Mongolian horse.BMC genetics · 2020Article
- HIPK4 is essential for murine spermiogenesis.eLife · 2020Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The molecular mechanisms regulating the spectacular cytodifferentiation observed during spermiogenesis are poorly understood. We have recently identified a murine testis-specific serine kinase (tssk) 1, constituting a novel subfamily of serine/threonine kinases. Using low stringency screening we have isolated and molecularly characterized a second closely related family member, tssk 2, which is probably the orthologue of the human DGS-G gene. Expression of tssk 1 and tssk 2 was limited to the testis of sexually mature males. Immunohistochemical staining localized both kinases to the cytoplasm of late spermatids and to structures resembling residual bodies. tssk 1 and tssk 2 were absent in released sperms in the lumen of the seminiferous tubules and the epididymis, demonstrating a tight window of expression restricted to the last stages of spermatid maturation. In vitro kinase assays of immunoprecipitates containing either tssk 1 or tssk 2 revealed no autophosphorylation of the kinases, however, they led to serine phosphorylation of a coprecipitating protein of approximately 65 kD. A search for interacting proteins using the yeast two-hybrid system with tssk 1 and tssk 2 cDNA as baits and a prey cDNA library from mouse testis, led to the isolation of a novel cDNA, interacting specifically with both tssk 1 and tssk 2, and encoding the coprecipitated 65-kD protein phosphorylated by both kinases. Interestingly, expression of the interacting clone was also testis specific and paralleled the developmental expression observed for the kinases themselves. These results represent the first demonstration of the involvement of a distinct kinase family, the tssk serine/threonine kinases, together with a substrate in the cytodifferentiation of late spermatids to sperms.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.