ArticleThe Journal of cell biology1997
Ultrastructural organization of bovine chromaffin cell cortex-analysis by cryofixation and morphometry of aspects pertinent to exocytosis.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 70 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
70 citing papers in PubMed.
- Profiling Endogenous Opioid Peptide Release from Adrenal Chromaffin Cells.ACS chemical neuroscience · 2026Article
- NSF is required for diverse endocytic modes by promoting fusion and fission pore closure in secretory cells.iScience · 2026Article
- Dense-core vesicles contain exosomes in secretory cells.Biophysical journal · 2025Article
- Clathrin mediates membrane fission and budding by constricting membrane pores.Cell discovery · 2024Article
- Advanced Imaging Techniques for the Characterization of Subcellular Organelle Structure in Pancreatic Islet β Cells.Comprehensive Physiology · 2023Article
- Untapped Potential: Real-Time Measurements of Opioid Exocytosis at Single Cells.Journal of the American Chemical Society · 2023Article
- Three membrane fusion pore families determine the pathway to pore dilation.Biophysical journal · 2023Article
- Exploiting Microelectrode Geometry for Comprehensive Detection of Individual Exocytosis Events at Single Cells.ACS sensors · 2023Article
- Technologies for Vitrification Based Cryopreservation.Bioengineering (Basel, Switzerland) · 2023Review
- VAMP2 and synaptotagmin mobility in chromaffin granule membranes: implications for regulated exocytosis.Molecular biology of the cell · 2022Article
- 5 ns electric pulses induce CaBioelectrochemistry (Amsterdam, Netherlands) · 2021Article
- The nanoscale molecular morphology of docked exocytic dense-core vesicles in neuroendocrine cells.Nature communications · 2021Article
- Synaptotagmin-7 places dense-core vesicles at the cell membrane to promote Munc13-2- and CaeLife · 2021Article
- Correlating Molecule Count and Release Kinetics with Vesicular Size Using Open Carbon Nanopipettes.Journal of the American Chemical Society · 2020Article
- Isolation of large dense-core vesicles from bovine adrenal medulla for functional studies.Scientific reports · 2020Article
- Carbon-Fiber Nanoelectrodes for Real-Time Discrimination of Vesicle Cargo in the Native Cellular Environment.ACS nano · 2020Article
- Vesicle Shrinking and Enlargement Play Opposing Roles in the Release of Exocytotic Contents.Cell reports · 2020Article
- Imaging FITC-dextran as a Reporter for Regulated Exocytosis.Journal of visualized experiments : JoVE · 2018Article
- Neurosecretion: what can we learn from chromaffin cells.Pflugers Archiv : European journal of physiology · 2018Review
- Adrenal Chromaffin Cells Exposed to 5-ns Pulses Require Higher Electric Fields to Porate Intracellular Membranes than the Plasma Membrane: An Experimental and Modeling Study.The Journal of membrane biology · 2017Article
10 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have analyzed ultrathin sections from isolated bovine chromaffin cells grown on plastic support, after fast freezing, by quantitative electron microscopy. We determined the size and intracellular distribution of dense core vesicles (DVs or chromaffin granules) and of clear vesicles (CVs). The average diameter of DVs is 356 nm, and that of CVs varies between 35-195 nm (average 90 nm). DVs appear randomly packed inside cells. When the distance of the center of DVs to the cell membrane (CM) is analyzed, DV density is found to decrease as the CM is approached. According to Monte Carlo simulations performed on the basis of the measured size distribution of DVs, this decay can be assigned to a "wall effect." Any cortical barrier, regardless of its function, seems to not impose a restriction to a random cortical DV packing pattern. The number of DVs closely approaching the CM (docked DVs) is estimated to be between 364 and 629 (average 496), i.e., 0.45 to 0.78 DVs/micron2 CM. Deprivation of Ca2+, priming by increasing [Ca2+]i, or depolarization by high [K+]e for 10 s (the effect of which was controlled electrophysiologically and predicted to change the number of readily releasable granules [RRGs]) does not significantly change the number of peripheral DVs. The reason may be that (a) structural docking implies only in part functional docking (capability of immediate release), and (b) exocytosis is rapidly followed by endocytosis and replenishment of the pool of docked DVs. Whereas the potential contribution of DVs to CM area increase by immediate release can be estimated at 19-33%, that of CVs is expected to be in the range of 5.6-8.0%.
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