ArticleThe Journal of clinical investigation1997
Tissue factor expression in human arterial smooth muscle cells. TF is present in three cellular pools after growth factor stimulation.
Article in The Journal of clinical investigation, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 146 citations in OpenAlex.
- Impact of an Alu insertion on the cellular localisation of tissue factor protein.Scientific reports · 2025Article
- Reduced blood EPAC1 protein levels as a marker of severe coronary artery disease: the role of hypoxic foam cell-transformed smooth muscle cells.Journal of translational medicine · 2025Article
- Endothelial-derived microvesicles promote pro-migratory cross-talk with smooth muscle cells by a mechanism requiring tissue factor and PAR2 activation.Frontiers in cardiovascular medicine · 2024Article
- C-Reactive Protein Signaling Pathways in Tumor Progression.Biomolecules & therapeutics · 2023Review
- Developing Biomimetic Hydrogels of the Arterial Wall as a Prothrombotic Substrate for In Vitro Human Thrombosis Models.Gels (Basel, Switzerland) · 2023Article
- Inflammation in Coronary Atherosclerosis and Its Therapeutic Implications.Cardiovascular drugs and therapy · 2022Review
- Exosomes and Atherogenesis.Frontiers in cardiovascular medicine · 2021Review
- Beyond thrombosis: the impact of tissue factor signaling in cancer.Journal of hematology & oncology · 2020Review
- A unique protein kinase C-dependent pathway for tissue factor downregulation in pericytes.Journal of thrombosis and haemostasis : JTH · 2019Article
- PDGF Restores the Defective Phenotype of Adipose-Derived Mesenchymal Stromal Cells from Diabetic Patients.Molecular therapy : the journal of the American Society of Gene Therapy · 2018Article
- Therapeutic Antibody-Like Immunoconjugates against Tissue Factor with the Potential to Treat Angiogenesis-Dependent as Well as Macrophage-Associated Human Diseases.Antibodies (Basel, Switzerland) · 2018Article
- Gene expression profiles and signaling mechanisms in αBMC systems biology · 2017Article
- Emerging roles for vascular smooth muscle cell exosomes in calcification and coagulation.The Journal of physiology · 2016Review
- Novel hydroxybutyl chitosan nanoparticles for siRNA delivery targeting tissue factor inhibits proliferation and induces apoptosis in human vascular smooth muscle cells.Molecular medicine reports · 2015Article
- Role of the protein C receptor in cancer progression.Thrombosis research · 2014Review
- Host cell invasion by Staphylococcus aureus stimulates the shedding of microvesicles.Biochemical and biophysical research communications · 2013Article
- Uremic serum and solutes increase post-vascular interventional thrombotic risk through altered stability of smooth muscle cell tissue factor.Circulation · 2013Article
- Therapeutic modulation of coagulation and fibrinolysis in acute lung injury and the acute respiratory distress syndrome.Current pharmaceutical biotechnology · 2011Review
- Tissue factor-bearing microparticles and thrombus formation.Arteriosclerosis, thrombosis, and vascular biology · 2011Review
- The endothelial protein C receptor supports tissue factor ternary coagulation initiation complex signaling through protease-activated receptors.The Journal of biological chemistry · 2011Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
Tissue factor (TF) is a transmembrane glycoprotein that initiates the coagulation cascade. Because of the potential role of TF in mediating arterial thrombosis, we have examined its expression in human aortic and coronary artery smooth muscle cells (SMC). TF mRNA and protein were induced in SMC by a variety of growth agonists. Exposure to PDGF AA or BB for 30 min provided all of the necessary signals for induction of TF mRNA and protein. This result was consistent with nuclear runoff analyses, demonstrating that PDGF-induced TF transcription occurred within 30 min. A newly developed assay involving binding of digoxigenin-labeled FVIIa (DigVIIa) and digoxigenin-labeled Factor X (DigX) was used to localize cellular TF. By light and confocal microscopy, prominent TF staining was seen in the perinuclear cytoplasm beginning 2 h after agonist treatment and persisting for 10-12 h. Surface TF activity, measured on SMC monolayers under flow conditions, increased transiently, peaking 4-6 h after agonist stimulation and returning to baseline within 16 h. Peak surface TF activity was only approximately 20% of total TF activity measured in cell lysates. Surface TF-blocking experiments demonstrated that the remaining TF was found as encrypted surface TF, and also in an intracellular pool. The relatively short-lived surface expression of TF may be critical for limiting the thrombotic potential of intact SMC exposed to growth factor stimulation. In contrast, the encrypted surface and intracellular pools may provide a rich source of TF under conditions associated with SMC damage, such as during atherosclerotic plaque rupture or balloon arterial injury.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.