ArticleGenetics1997
Genetic analysis of the Chlamydomonas reinhardtii I-CreI mobile intron homing system in Escherichia coli.
Article in Genetics, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- A multifunctional system for genome editing and large-scale interspecies gene transfer.Nature communications · 2022Article
- Crystal Structure of the Homing Endonuclease I-CvuI Provides a New Template for Genome Modification.The Journal of biological chemistry · 2015Article
- Targeted DNA excision in Arabidopsis by a re-engineered homing endonuclease.BMC biotechnology · 2012Article
- Comprehensive homing endonuclease target site specificity profiling reveals evolutionary constraints and enables genome engineering applications.Nucleic acids research · 2012Article
- The mitochondrial LSU rRNA group II intron of Ustilago maydis encodes an active homing endonuclease likely involved in intron mobility.PloS one · 2012Article
- Molecular basis of engineered meganuclease targeting of the endogenous human RAG1 locus.Nucleic acids research · 2011Article
- The Agaricus bisporus cox1 gene: the longest mitochondrial gene and the largest reservoir of mitochondrial group i introns.PloS one · 2010Article
- Homing endonucleases: from basics to therapeutic applications.Cellular and molecular life sciences : CMLS · 2010Review
- Generation of single-chain LAGLIDADG homing endonucleases from native homodimeric precursor proteins.Nucleic acids research · 2009Article
- Article
- Genetic selection system for improving recombinant membrane protein expression in E. coli.Protein science : a publication of the Protein Society · 2009Article
- Engineering variants of the I-SceI homing endonuclease with strand-specific and site-specific DNA-nicking activity.Journal of molecular biology · 2008Article
- The C-terminal loop of the homing endonuclease I-CreI is essential for site recognition, DNA binding and cleavage.Nucleic acids research · 2007Article
- Homing endonuclease I-CreI derivatives with novel DNA target specificities.Nucleic acids research · 2006Article
- In vivo selection of engineered homing endonucleases using double-strand break induced homologous recombination.Nucleic acids research · 2005Article
- Article
- A novel engineered meganuclease induces homologous recombination in yeast and mammalian cells.Nucleic acids research · 2003Article
- Mutations altering the cleavage specificity of a homing endonuclease.Nucleic acids research · 2002Article
- Targeted mutagenesis by homologous recombination in D. melanogaster.Genes & development · 2002Article
- An in vivo selection system for homing endonuclease activity.Nucleic acids research · 2002Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
We have developed and used a genetic selection system in Escherichia coli to study functional requirements for homing site recognition and cleavage by a representative eukaryotic mobile intron endonuclease. The homing endonuclease, I-CreI, was originally isolated from the chloroplast of the unicellular green alga Chlamydomonas reinhardtii. I-CreI homing site mutants contained base pair substitutions or single base deletions that altered the rate of homing site cleavage and/or product release. I-CreI endonuclease mutants fell into six phenotypic classes that differed in in vivo activity, toxicity or genetic dominance. Inactivating mutations clustered in the N-terminal 60% of the I-CreI amino acid sequence, and two frameshift mutations were isolated that resulted in premature translation termination though retained partial activity. These mutations indicate that the N-terminal two-thirds of the I-CreI endonuclease is sufficient for homing site recognition and cleavage. Substitution mutations altered in four potential active site residues were examined: D20N, Q47H or R70A substitutions inactivated endonuclease activity, whereas S22A did not. The genetic approach we have taken complements phylogenetic and structural studies of mobile intron endonucleases and has provided new information on the mechanistic basis of I-CreI homing site recognition and cleavage.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.