ArticleGenetics1997
The yeast HRS1 gene is involved in positive and negative regulation of transcription and shows genetic characteristics similar to SIN4 and GAL11.
Article in Genetics, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 51 citations in OpenAlex.
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- Mediator tail subunits can form amyloid-like aggregates in vivo and affect stress response in yeast.Nucleic acids research · 2015Article
- Facilitated assembly of the preinitiation complex by separated tail and head/middle modules of the mediator.Journal of molecular biology · 2012Article
- The tail-module of yeast Mediator complex is required for telomere heterochromatin maintenance.Nucleic acids research · 2012Article
- New suppressors of THO mutations identify Thp3 (Ypr045c)-Csn12 as a protein complex involved in transcription elongation.Molecular and cellular biology · 2011Article
- A reduction in RNA polymerase II initiation rate suppresses hyper-recombination and transcription-elongation impairment of THO mutants.Molecular genetics and genomics : MGG · 2008Article
- A functional module of yeast mediator that governs the dynamic range of heat-shock gene expression.Genetics · 2006Article
- Independent recruitment of mediator and SAGA by the activator Met4.Molecular and cellular biology · 2006Article
- Genetic interactions of DST1 in Saccharomyces cerevisiae suggest a role of TFIIS in the initiation-elongation transition.Genetics · 2004Article
- A multiplicity of coactivators is required by Gcn4p at individual promoters in vivo.Molecular and cellular biology · 2003Article
- Role of transcription in plasmid maintenance in the hpr1Delta mutant of Saccharomyces cerevisiae.Molecular and cellular biology · 2002Article
- Restoration of silencing in Saccharomyces cerevisiae by tethering of a novel Sir2-interacting protein, Esc8.Genetics · 2002Article
- The yeast protein Xtc1 functions as a direct transcriptional repressor.Nucleic acids research · 2002Article
- hpr1Delta affects ribosomal DNA recombination and cell life span in Saccharomyces cerevisiae.Molecular and cellular biology · 2002Article
- The Swi5 activator recruits the Mediator complex to the HO promoter without RNA polymerase II.Genes & development · 2001Article
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- Srb7p is a physical and physiological target of Tup1p.The EMBO journal · 2000Article
- In vivo requirement of activator-specific binding targets of mediator.Molecular and cellular biology · 2000Article
- An activator binding module of yeast RNA polymerase II holoenzyme.Molecular and cellular biology · 1999Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We provide genetic evidence that HRS1/PGD1, a yeast gene previously identified as a suppressor of the hyper-recombination phenotype of hpr1, has positive and negative roles in transcriptional regulation. We have analyzed three differently regulated promoters, GAL1, PHO5 and HSP26, by beta-galactosidase assays of lacZ-fused promoters and by Northern analysis of the endogenous genes. Transcription of these promoters was derepressed in hrs1delta mutants under conditions in which it is normally repressed in wild type. Under induced conditions it was either strongly reduced or significantly enhanced depending on the promoter system analyzed. Constitutive transcription was not affected, as determined in ADH1 and TEF2. In addition, Hrs1p was required for mating-factor expression, telomere-linked DNA silencing and DNA supercoiling of plasmids. Furthermore, hrs1delta suppressed Ty-insertion mutations and conferred a Gal- phenotype. Many of these phenotypes also result from mutations in GAL11, SIN4 or RGR1, which encode proteins of the RNA polII mediator. We also show that gal11delta and sin4delta partially suppress the hyper-rec phenotype of hpr1 mutants, although to a lesser extent than hrs1delta. Our results provide new evidence for the connection between hpr1delta-induced deletions and transcription. We discuss the possibility that Hrs1p might be a component of the RNA polII transcription machinery.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.