ArticleThe Journal of physiology1997
Ca2+ current and Ca(2+)-activated chloride current in isolated smooth muscle cells of the sheep urethra.
Article in The Journal of physiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 63 citations in OpenAlex.
- What do we really know about the external urethral sphincter?Continence (Amsterdam, Netherlands) · 2024Review
- CaPhysiological reports · 2022Article
- Lymphatic Collecting Vessel: New Perspectives on Mechanisms of Contractile Regulation and Potential Lymphatic Contractile Pathways to Target in Obesity and Metabolic Diseases.Frontiers in pharmacology · 2022Review
- Anoctamin 1 antagonism potentiates conventional tocolytic-mediated relaxation of pregnant human uterine smooth muscle.The journal of physiological sciences : JPS · 2021Article
- Functional comparison of anoctamin 1 antagonists on human uterine smooth muscle contractility and excitability.Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi · 2018Article
- Effects of new-generation TMEM16A inhibitors on calcium-activated chloride currents in rabbit urethral interstitial cells of Cajal.Pflugers Archiv : European journal of physiology · 2017Article
- 9-Anthracene carboxylic acid is more suitable than DIDS for characterization of calcium-activated chloride current during canine ventricular action potential.Naunyn-Schmiedeberg's archives of pharmacology · 2015Article
- Interstitial cells: regulators of smooth muscle function.Physiological reviews · 2014Review
- Pharmacological characterization of TMEM16A currents.Channels (Austin, Tex.) · 2014Article
- Ion channels of the mammalian urethra.Channels (Austin, Tex.) · 2014Review
- Contribution of Kv2.1 channels to the delayed rectifier current in freshly dispersed smooth muscle cells from rabbit urethra.American journal of physiology. Cell physiology · 2011Article
- Heterogeneous CPA sensitivity of spontaneous excitation in smooth muscle of the rabbit urethra.British journal of pharmacology · 2006Article
- Mefenamic acid as a novel activator of L-type voltage-dependent Ca2+ channels in smooth muscle cells from pig proximal urethra.British journal of pharmacology · 2005Article
- Modulation of spontaneous Ca2+-activated Cl- currents in the rabbit corpus cavernosum by the nitric oxide-cGMP pathway.The Journal of physiology · 2004Article
- Electrophysiological characterization and functional importance of calcium-activated chloride channel in rat uterine myocytes.Pflugers Archiv : European journal of physiology · 2004Article
- The Cl(-) channel blocker niflumic acid releases Ca(2+) from an intracellular store in rat pulmonary artery smooth muscle cells.British journal of pharmacology · 2003Article
- Role of sarcoplasmic reticulum in control of membrane potential and nitrergic response in opossum lower esophageal sphincter.British journal of pharmacology · 2003Article
- T- and L-type Ca2+ currents in freshly dispersed smooth muscle cells from the human proximal urethra.The Journal of physiology · 2003Article
- Anomalous effect of anthracene-9-carboxylic acid on calcium-activated chloride currents in rabbit pulmonary artery smooth muscle cells.British journal of pharmacology · 2003Article
- The involvement of L-type Ca(2+) channels in the relaxant effects of the ATP-sensitive K(+) channel opener ZD6169 on pig urethral smooth muscle.British journal of pharmacology · 2001Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
1. Isolated sheep urethral cells were studied using the perforated patch clamp technique (T = 37 degrees C). Depolarizing steps ranging from -40 to -10 mV evoked an inward current that peaked within 10 ms and a slower inward current. Stepping back to the holding potential of -80 mV evoked large inward tail currents. All three currents were abolished by nifedipine (1 microM). Substitution of external Ca2+ with Ba2+ resulted in potentiation of the fast inward current and blockade of the slow current and tails. 2. Changing the chloride equilibrium potential (ECl) from 0 to +27 mV shifted the reversal potential of the tail currents from 1 +/- 1 to 27 +/- 1 mV (number of cells, n = 5). Chloride channel blockers, niflumic acid (10 microM) and anthracene-9-carboxylic acid (9AC, 1 mM), reduced the slow current and tails suggesting that these were Ca(2+)-activated Cl- currents, ICl(Ca). 4. Caffeine (10 mM) induced currents that reversed at ECl and were blocked by niflumic acid (10 microM). 5. In current clamp mode, some cells developed spontaneous transient depolarizations (STDs) and action potentials. Short exposure to nifedipine blocked the action potentials and unmasked STDs. In contrast, 9AC and niflumic acid reduced the amplitude of the STDs and blocked the action potentials. 6. In conclusion, these cells have both L-type ICa and ICl(Ca). The former appears to be responsible for the upstroke of the action potential, while the latter may act as a pacemaker current.
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