ArticleThe Journal of physiology1997
Characterization of a nicotinamide-adenine dinucleotide-dependent cation channel in the CRI-G1 rat insulinoma cell line.
Article in The Journal of physiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 41 citations in OpenAlex.
- Characterization and Optimization of the Novel Transient Receptor Potential Melastatin 2 Antagonist tatM2NX.Molecular pharmacology · 2020Article
- The overexpressed functional transient receptor potential channel TRPM2 in oral squamous cell carcinoma.Scientific reports · 2016Article
- Androgen and PARP-1 regulation of TRPM2 channels after ischemic injury.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2013Article
- Regulation of ion channels by pyridine nucleotides.Circulation research · 2013Review
- TRPM2 channel activation following in vitro ischemia contributes to male hippocampal cell death.Neuroscience letters · 2012Article
- Metabolic stress, reactive oxygen species, and arrhythmia.Journal of molecular and cellular cardiology · 2012Review
- TRP channels and their implications in metabolic diseases.Pflugers Archiv : European journal of physiology · 2011Review
- Activation of store-operated I(CRAC) by hydrogen peroxide.Cell calcium · 2010Article
- Modulation of TRPM2 by acidic pH and the underlying mechanisms for pH sensitivity.The Journal of general physiology · 2009Article
- Cardiac Na+ current regulation by pyridine nucleotides.Circulation research · 2009Article
- Synergistic regulation of endogenous TRPM2 channels by adenine dinucleotides in primary human neutrophils.Cell calcium · 2008Article
- Regulation of TRPM2 by extra- and intracellular calcium.The Journal of general physiology · 2007Article
- The role of TRPM channels in cell death.Pflugers Archiv : European journal of physiology · 2005Review
- Hydrogen-peroxide-induced toxicity of rat striatal neurones involves activation of a non-selective cation channel.The Journal of physiology · 2003Article
- Reduced glutathione inhibits beta-NAD+-activated non-selective cation currents in the CRI-G1 rat insulin-secreting cell line.The Journal of physiology · 1999Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
1. Cell-free excised membrane patches were used to examine the properties of a novel nicotinamide-adenine dinucleotide (beta-NAD+)-activated ion channel in the rat insulin-secreting cell line, CRI-G1. 2. In inside-out recordings, beta-NAD+ (0.05-1.0 mM) induced the appearance of a channel characterized by extremely slow kinetics, with mean open times in the range of seconds. The estimated EC50 for activation was 114 microM. Channel activity declined with time (run-down) following activation by beta-NAD+ in excised patches and this was not prevented by intracellular application of trypsin. 3. The single channel current-voltage relationship was linear with a conductance of 74 pS in symmetrical NaCl. The channel appears equally permeable to Na+, K+ and Cs+, exhibits an appreciable permeability to Ca2+, Mg2+ and Ba2+, but excludes anions. 4. The channel displays an unusual voltage sensitivity, with an abrupt increase in open-state probability at depolarized voltages. 5. Channel opening, in the presence of beta-NAD+, required both Ca2+ and Mg2+ to be present at the internal side of the membrane. Activation by Ca2+ required a concentration of at least 10 microM and was maximal at 0.1 mM. Ba2+ did not substitute for Ca2+ in inducing channel activity nor did it inhibit activation by Ca2+. Increasing the concentration of intracellular Mg2+ stabilized the open state of NAD(+)-activated channels. 6. The non-selective cation channel reported here differs in its gating and modulatory characteristics from non-selective cation channels described in other tissues. This channel may play a role in the pathophysiological responses of beta-cells to oxidative stress.
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