ArticleGenes & development1997
The yeast HPR1 gene has a functional role in transcriptional elongation that uncovers a novel source of genome instability.
Article in Genes & development, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 103 papers.
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Who cites it
103 citing papers in PubMed, 162 citations in OpenAlex.
- A yeast recombination system recapitulates the length polymorphism of a microsatellite implicated in human social behavior.NAR molecular medicine · 2026Article
- DNA lesions can frequently precede DNA:RNA hybrid accumulation.Nature communications · 2025Article
- Expression of human RECQL5 in Saccharomyces cerevisiae causes transcription defects and transcription-associated genome instability.Molecular genetics and genomics : MGG · 2024Article
- Rat1 promotes premature transcription termination at R-loops.Nucleic acids research · 2024Article
- The CSB chromatin remodeler regulates PARP1- and PARP2-mediated single-strand break repair at actively transcribed DNA regions.Nucleic acids research · 2023Article
- The THO complex counteracts TERRA R-loop-mediated telomere fragility in telomerase+ cells and telomeric recombination in ALT+ cells.Nucleic acids research · 2023Article
- Detection of R-Loops by In Vivo and In Vitro Cytosine Deamination in Saccharomyces cerevisiae.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Harmful R-loops are prevented via different cell cycle-specific mechanisms.Nature communications · 2021Article
- Transcription-Replication Collisions and Chromosome Fragility.Frontiers in genetics · 2021Review
- R-loops at centromeric chromatin contribute to defects in kinetochore integrity and chromosomal instability in budding yeast.Molecular biology of the cell · 2021Article
- Review
- Harmful DNA:RNA hybrids are formedeLife · 2020Article
- Histone H3E73Q and H4E53A mutations cause recombinogenic DNA damage.Microbial cell (Graz, Austria) · 2020Article
- Rpd3L and Hda1 histone deacetylases facilitate repair of broken forks by promoting sister chromatid cohesion.Nature communications · 2019Article
- Cdc73 suppresses genome instability by mediating telomere homeostasis.PLoS genetics · 2018Article
- Regulation of transcription elongation in response to osmostress.PLoS genetics · 2017Article
- Physical proximity of chromatin to nuclear pores prevents harmful R loop accumulation contributing to maintain genome stability.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Review
- Review
- Transcription-replication conflicts at chromosomal fragile sites-consequences in M phase and beyond.Chromosoma · 2017Review
43 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The yeast HPR1 gene plays an important role in genome stability, as indicated by the observation that hpr1 mutants have high frequencies of DNA repeat recombination and chromosome loss. Here we report that HPR1 is required for transcriptional elongation. Transcription driven from constitutive and regulated yeast promoters cannot elongate through the bacterial lacZ coding region in hpr1Delta cells, but progresses efficiently through other sequences such as yeast PHO5. We show that HPR1 is not required for transcription activation and that the previously reported effects of hpr1Delta on the activation of different promoters is a consequence of the incapacity of hpr1Delta cells to elongate transcription through lacZ, used as reporter. Transcriptional defects are also observed in yeast DNA sequences of hpr1Delta cells in the presence of the transcription elongation inhibitor 6-azauracil. In all cases, the blockage of transcription elongation in hpr1Delta is associated with both the high frequency of deletions and the increase in plasmid instability that we report here. Therefore, in addition to the identification of a new element involved in transcriptional elongation, our work provides evidence for a new source of genomic instability.
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