ArticleProceedings of the National Academy of Sciences of the United States of America1997
Protein kinase A activity is required for the budding of constitutive transport vesicles from the trans-Golgi network.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 97 citations in OpenAlex.
- Article
- PKA Activity-Driven Modulation of Bidirectional Long-Distance transport of Lysosomal vesicles During Synapse Maintenance.bioRxiv : the preprint server for biology · 2024Article
- Human adenylyl cyclase 9 is auto-stimulated by its isoform-specific C-terminal domain.Life science alliance · 2023Article
- cAMP-specific phosphodiesterase 8A and 8B isoforms are differentially expressed in human testis and Leydig cell tumor.Frontiers in endocrinology · 2022Article
- The Small Molecule H89 InhibitsInfection and immunity · 2021Article
- The Golgi complex: a hub of the secretory pathway.BMB reports · 2021Review
- Protein kinase D and Gβγ mediate sustained nociceptive signaling by biased agonists of protease-activated receptor-2.The Journal of biological chemistry · 2019Article
- PKAc is not required for the preerythrocytic stages ofLife science alliance · 2019Article
- OPA1-anchored PKA phosphorylates perilipin 1 on S522 and S497 in adipocytes differentiated from human adipose stem cells.Molecular biology of the cell · 2018Article
- Targeted protein unfolding uncovers a Golgi-specific transcriptional stress response.Molecular biology of the cell · 2018Article
- Protein kinase A activity is necessary for fission and fusion of Golgi to endoplasmic reticulum retrograde tubules.PloS one · 2015Article
- Regulation of Golgi signaling and trafficking by the KDEL receptor.Histochemistry and cell biology · 2013Review
- Stimulation of norepinephrine transporter function by fasudil, a Rho kinase inhibitor, in cultured bovine adrenal medullary cells.Naunyn-Schmiedeberg's archives of pharmacology · 2012Article
- AKAP350 Is involved in the development of apical "canalicular" structures in hepatic cells HepG2.Journal of cellular physiology · 2012Article
- Unconventional secretion of Ebola virus matrix protein VP40.The Journal of infectious diseases · 2011Article
- Signaling at the Golgi.Cold Spring Harbor perspectives in biology · 2011Review
- Sugar-free frosting, a homolog of SAD kinase, drives neural-specific glycan expression in the Drosophila embryo.Development (Cambridge, England) · 2011Article
- Mechanoregulation of BK channel activity in the mammalian cortical collecting duct: role of protein kinases A and C.American journal of physiology. Renal physiology · 2009Article
- Proteasome inhibition enhances the induction and impairs the maintenance of late-phase long-term potentiation.Learning & memory (Cold Spring Harbor, N.Y.) · 2008Article
- Dopamine and ethanol cause translocation of epsilonPKC associated with epsilonRACK: cross-talk between cAMP-dependent protein kinase A and protein kinase C signaling pathways.Molecular pharmacology · 2008Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have examined the role played by protein kinase A (PKA) in vesicle-mediated protein transport from the trans-Golgi network (TGN) to the cell surface. In vivo this transport step was inhibited by inhibitors of PKA catalytic subunits (C-PKA) such as the compound known as H89 and a myristoylated form of the inhibitory peptide sequence contained in the thermostable PKA inhibitor. Inhibition by H89 occurred at an early stage during the transfer of vesicular stomatitis virus G glycoprotein from the TGN to the cell surface. Reversal from this inhibition correlated with a transient increase in the number of free coated vesicles in the Golgi area. Vesicle budding from the TGN was studied in vitro using vesicular stomatitis virus-infected, permeabilized cells. Addition to this assay of C-PKA stimulated vesicle release while it was suppressed by PKA inhibitory peptide, H89, and antibody against C-PKA. Furthermore, vesicle release was decreased when PKA-depleted cytosol was used and restored by addition of C-PKA. These results indicate a regulatory role for PKA activity in the production of constitutive transport vesicles from the TGN.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.