ArticleProceedings of the National Academy of Sciences of the United States of America1997
Inhibition of mRNA export in vertebrate cells by nuclear export signal conjugates.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 55 citations in OpenAlex.
- Ran modulates allosteric crosstalk between importin β surfaces.Nature communications · 2025Article
- Enhancement of gene silencing effect and membrane permeability by Peptide-conjugated 27-nucleotide small interfering RNA.Molecules (Basel, Switzerland) · 2012Article
- Yeast poly(A)-binding protein, Pab1, and PAN, a poly(A) nuclease complex recruited by Pab1, connect mRNA biogenesis to export.Genes & development · 2005Article
- Coordinated nuclear export of 60S ribosomal subunits and NMD3 in vertebrates.The EMBO journal · 2003Article
- Identification of a novel posttranscriptional regulatory element by using a rev- and RRE-mutated human immunodeficiency virus type 1 DNA proviral clone as a molecular trap.Journal of virology · 2001Article
- The nuclear export receptor Xpo1p forms distinct complexes with NES transport substrates and the yeast Ran binding protein 1 (Yrb1p).Molecular biology of the cell · 2001Article
- Protein ligands to HuR modulate its interaction with target mRNAs in vivo.The Journal of cell biology · 2000Article
- Nuclear export of heat shock and non-heat-shock mRNA occurs via similar pathways.Molecular and cellular biology · 2000Article
- Yeast Ran-binding protein 1 (Yrb1) shuttles between the nucleus and cytoplasm and is exported from the nucleus via a CRM1 (XPO1)-dependent pathway.Molecular and cellular biology · 2000Article
- Facilitated nucleocytoplasmic shuttling of the Ran binding protein RanBP1.Molecular and cellular biology · 2000Article
- Splicing is required for rapid and efficient mRNA export in metazoans.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- RanGTP-regulated interactions of CRM1 with nucleoporins and a shuttling DEAD-box helicase.Molecular and cellular biology · 1999Article
- Uncoupling of the hnRNP Npl3p from mRNAs during the stress-induced block in mRNA export.Genes & development · 1999Article
- A Crm1p-independent nuclear export path for the mRNA-associated protein, Npl3p/Mtr13p.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- CRM1-mediated recycling of snurportin 1 to the cytoplasm.The Journal of cell biology · 1999Article
- An exon that prevents transport of a mature mRNA.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- The nucleoporin nup153 plays a critical role in multiple types of nuclear export.Molecular biology of the cell · 1999Article
- Crm1p mediates regulated nuclear export of a yeast AP-1-like transcription factor.The EMBO journal · 1998Article
- Mta has properties of an RNA export protein and increases cytoplasmic accumulation of Epstein-Barr virus replication gene mRNA.Journal of virology · 1998Article
- Yeast Los1p has properties of an exportin-like nucleocytoplasmic transport factor for tRNA.Molecular and cellular biology · 1998Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Leucine-rich nuclear export signals (NESs) are recognized by the NES receptor exportin 1 and are central to the export of multiple shuttling proteins and RNAs. The export of messenger RNA in vertebrates was, however, thought to occur by a different pathway, because inhibition by injection of a synthetic Rev NES conjugate could not be demonstrated. Here we find that peptide conjugates composed of the NES of either protein kinase A inhibitor protein (PKI) or the HIV-1 Rev protein, when coupled to human serum albumin, are potent inhibitors of mRNA and small nuclear RNA export. These results provide direct evidence that mRNA export in vertebrates depends on interactions between an NES and its cognate NES receptors. PKI NES conjugates are significantly more efficient at inhibiting RNA export than are REV NES conjugates, indicating that different NESs may have different abilities to promote protein and RNA export. Surprisingly, an expected control conjugate containing the mutant Rev NES sequence M10 strongly inhibited the export of intronless dihydrofolate reductase mRNA. Nuclear injection of NES peptide conjugates led to mislocalization to the nucleus of 10-20% of the cytoplasmic Ran GTPase-binding protein (RanBP1) indicating that RanBP1 shuttles between the nucleus and the cytoplasm via an NES pathway. These results demonstrate that in vertebrates the export of mRNA, like that of small nuclear RNA, 5S rRNA, and transport factors such as RanBP1, employs NES-mediated molecular machinery.
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