ArticleThe EMBO journal1997
Position-dependent and promoter-specific regulation of gene expression in Trypanosoma brucei.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
36 citing papers in PubMed, 78 citations in OpenAlex.
- Unwrap RAP1's Mystery at Kinetoplastid Telomeres.Biomolecules · 2024Review
- Article
- TbSAP is a novel chromatin protein repressing metacyclic variant surface glycoprotein expression sites in bloodstream form Trypanosoma brucei.Nucleic acids research · 2021Article
- Keeping Balance Between Genetic Stability and Plasticity at the Telomere and Subtelomere ofFrontiers in cell and developmental biology · 2021Review
- Temperature shift activates bloodstream VSG expression site promoters in Trypanosoma brucei.Molecular and biochemical parasitology · 2018Article
- The Structure of a Conserved Telomeric Region Associated with Variant Antigen Loci in the Blood Parasite Trypanosoma congolense.Genome biology and evolution · 2018Article
- Simplified inducible system for Trypanosoma brucei.PloS one · 2018Article
- VEX1 controls the allelic exclusion required for antigenic variation in trypanosomes.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Inositol phosphate pathway controls transcription of telomeric expression sites in trypanosomes.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Review
- Locus-specific control of DNA resection and suppression of subtelomeric VSG recombination by HAT3 in the African trypanosome.Nucleic acids research · 2014Article
- Antigenic variation in African trypanosomes.Molecular and biochemical parasitology · 2014Review
- Article
- Telomere components as potential therapeutic targets for treating microbial pathogen infections.Frontiers in oncology · 2012Article
- Histone H1 plays a role in heterochromatin formation and VSG expression site silencing in Trypanosoma brucei.PLoS pathogens · 2012Article
- TbISWI regulates multiple polymerase I (Pol I)-transcribed loci and is present at Pol II transcription boundaries in Trypanosoma brucei.Eukaryotic cell · 2011Article
- Microhomology-mediated deletion and gene conversion in African trypanosomes.Nucleic acids research · 2011Article
- NLP is a novel transcription regulator involved in VSG expression site control in Trypanosoma brucei.Nucleic acids research · 2011Article
- Site-specific DNA double-strand breaks greatly increase stable transformation efficiency in Trypanosoma brucei.Molecular and biochemical parasitology · 2009Article
- RAP1 is essential for silencing telomeric variant surface glycoprotein genes in Trypanosoma brucei.Cell · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Trypanosoma brucei evades the mammalian immune response by a process of antigenic variation. This involves mutually exclusive and alternating expression of telomere-proximal variant surface glycoprotein genes (vsgs), which is controlled at the level of transcription. To examine transcription repression in T.brucei we inserted reporter genes, under the control of either rRNA or vsg expression site (ES) promoters, into various chromosomal loci. Position-dependent repression of both promoters was observed in the mammalian stage of the life cycle (bloodstream forms). Repression of promoters inserted into a silent ES was more pronounced closer to the telomere and was bi-directional. Transcription from both ES and rRNA promoters was also efficiently repressed at a non-telomeric vsg locus in bloodstream-form trypanosomes. In cultured tsetse fly midgut-stage (procyclic) trypanosomes, in which vsg is not normally expressed, all inserted rRNA promoters were derepressed but ES promoters remained silent. Our results suggest that vsg promoters and ectopic rRNA promoters in bloodstream-form T.brucei are restrained by position effects related to their proximity to vsgs or other features of the ES. Sequences present in rRNA promoters but absent from vsg ES promoters appear to be responsible for rRNA promoter-specific derepression in procyclic cells.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.