ArticleThe EMBO journal1997
Overexpression of Pex15p, a phosphorylated peroxisomal integral membrane protein required for peroxisome assembly in S.cerevisiae, causes proliferation of the endoplasmic reticulum membrane.
Article in The EMBO journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
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Who cites it
79 citing papers in PubMed.
- The yeast peroxisomal proteome at absolute quantitative scale.Histochemistry and cell biology · 2026Article
- Msp1 and Pex19-Pex3 cooperate to achieve correct localization of Pex15 to peroxisomes.The FEBS journal · 2025Article
- The subset of peroxisomal tail-anchored proteins do not reach peroxisomes via ER, instead mitochondria can be involved.PloS one · 2023Article
- Enhanced production of acetyl-CoA-based products via peroxisomal surface display inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Insights into the Structure and Function of the Pex1/Pex6 AAA-ATPase in Peroxisome Homeostasis.Cells · 2022Review
- Atg39 selectively captures inner nuclear membrane into lumenal vesicles for delivery to the autophagosome.The Journal of cell biology · 2021Article
- Post-translational modifications of proteins associated with yeast peroxisome membrane: An essential mode of regulatory mechanism.Genes to cells : devoted to molecular & cellular mechanisms · 2021Review
- The Pex3-Inp1 complex tethers yeast peroxisomes to the plasma membrane.The Journal of cell biology · 2020Article
- Inducible intracellular membranes: molecular aspects and emerging applications.Microbial cell factories · 2020Review
- The AAA+ ATPase Msp1 is a processive protein translocase with robust unfoldase activity.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Divergence of Peroxisome Membrane Gene Sequence and Expression Between Yeast Species.G3 (Bethesda, Md.) · 2020Article
- A Mechanistic Perspective on PEX1 and PEX6, Two AAA+ Proteins of the Peroxisomal Protein Import Machinery.International journal of molecular sciences · 2019Review
- Protein Topology Prediction Algorithms Systematically Investigated in the Yeast Saccharomyces cerevisiae.BioEssays : news and reviews in molecular, cellular and developmental biology · 2019Article
- The natural history of Get3-like chaperones.Traffic (Copenhagen, Denmark) · 2019Review
- Peroxisome biogenesis, membrane contact sites, and quality control.EMBO reports · 2019Review
- A bacteria-derived tail anchor localizes to peroxisomes in yeast and mammalian cells.Scientific reports · 2018Article
- The peroxisomal AAA-ATPase Pex1/Pex6 unfolds substrates by processive threading.Nature communications · 2018Article
- Peroxisome Function, Biogenesis, and Dynamics in Plants.Plant physiology · 2018Review
- A versatile plasmid system for reconstitution and analysis of mammalian ubiquitination cascades in yeast.Microbial cell (Graz, Austria) · 2017Article
- Disparate peroxisome-related defects in Arabidopsis pex6 and pex26 mutants link peroxisomal retrotranslocation and oil body utilization.The Plant journal : for cell and molecular biology · 2017Article
19 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors.
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Abstract
We have cloned PEX15 which is required for peroxisome biogenesis in Saccharomyces cerevisiae. pex15Delta cells are characterized by the cytosolic accumulation of peroxisomal matrix proteins containing a PTS1 or PTS2 import signal, whereas peroxisomal membrane proteins are present in peroxisomal remnants. PEX15 encodes a phosphorylated, integral peroxisomal membrane protein (Pex15p). Using multiple in vivo methods to determine the topology, Pex15p was found to be a tail-anchored type II (Ncyt-Clumen) peroxisomal membrane protein with a single transmembrane domain near its carboxy-terminus. Overexpression of Pex15p resulted in impaired peroxisome assembly, and caused profound proliferation of the endoplasmic reticulum (ER) membrane. The lumenal carboxy-terminal tail of Pex15p protrudes into the lumen of these ER membranes, as demonstrated by its O-glycosylation. Accumulation in the ER was also observed at an endogenous expression level when Pex15p was fused to the N-terminus of mature invertase. This resulted in core N-glycosylation of the hybrid protein. The lumenal C-terminal tail of Pex15p is essential for targeting to the peroxisomal membrane. Furthermore, the peroxisomal membrane targeting signal of Pex15p overlaps with an ER targeting signal on this protein. These results indicate that Pex15p may be targeted to peroxisomes via the ER, or to both organelles.
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