ArticleThe American journal of pathology1997
Sp1-mediated transactivation of LamC1 promoter and coordinated expression of laminin-gamma1 and Sp1 in human hepatocellular carcinomas.
Article in The American journal of pathology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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Who cites it
18 citing papers in PubMed, 44 citations in OpenAlex.
- LAMC1 upregulation via TGFβ induces inflammatory cancer-associated fibroblasts in esophageal squamous cell carcinoma via NF-κB-CXCL1-STAT3.Molecular oncology · 2021Article
- LAMC1 is a prognostic factor and a potential therapeutic target in endometrial cancer.Journal of gynecologic oncology · 2020Article
- The Many Microenvironments of Ovarian Cancer.Advances in experimental medicine and biology · 2020Review
- Identification of a transcriptomic signature with excellent survival prediction for squamous cell carcinoma of the cervix.American journal of cancer research · 2020Article
- High LAMC1 expression in glioma is associated with poor prognosis.OncoTargets and therapy · 2019Article
- Overexpression of LAMC1 predicts poor prognosis and enhances tumor cell invasion and migration in hepatocellular carcinoma.Journal of Cancer · 2017Article
- Article
- Laminin C1 expression by uterine carcinoma cells is associated with tumor progression.Gynecologic oncology · 2015Article
- MiR-1188 at the imprinted Dlk1-Dio3 domain acts as a tumor suppressor in hepatoma cells.Molecular biology of the cell · 2015Article
- The diagnostic and biological implications of laminin expression in serous tubal intraepithelial carcinoma.The American journal of surgical pathology · 2012Article
- Oncosuppressive role of p53-induced miR-205 in triple negative breast cancer.Molecular oncology · 2012Article
- Factors modulating expression of Renilla luciferase from control plasmids used in luciferase reporter gene assays.Analytical biochemistry · 2010Review
- Thiazolidinediones mimic glucose starvation in facilitating Sp1 degradation through the up-regulation of beta-transducin repeat-containing protein.Molecular pharmacology · 2009Article
- Article
- Kruppel-like factors regulate the Lama1 gene encoding the laminin alpha1 chain.The Journal of biological chemistry · 2004Article
- Synergistic activation of the rat laminin gamma1 chain promoter by the gut-enriched Kruppel-like factor (GKLF/KLF4) and Sp1.Nucleic acids research · 2002Article
- Involvement of activator protein 1 complexes in the epithelium-specific activation of the laminin gamma2-chain gene promoter by hepatocyte growth factor (scatter factor).The Biochemical journal · 2000Article
- Differential expression and origin of membrane-type 1 and 2 matrix metalloproteinases (MT-MMPs) in association with MMP2 activation in injured human livers.The American journal of pathology · 1998Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The laminin-gamma1 chain is present in most basement membranes and is involved in various physiological and pathological processes, including carcinogenesis in the liver. We have investigated the role of the transcription factor Sp1 in the activation of the LamC1 gene, which encodes laminin-gamma1, both in hepatocytes and in human hepatocellular carcinomas. DNAse I hypersensitive sites were mapped in the murine LamC1 promoter using early hepatocyte primary cultures in which LamC1 becomes activated. Three hypersensitive sites were found in enhancer-like elements that contain GC-rich regions. Gel-shift analyses showed that specific complexes were resolved using GC-containing oligonucleotides and Faza 567 hepatoma cells, which constitutively express laminin-gamma1 at a high level. Increased GC-binding activity was observed using nuclear extracts from early hepatocyte cultures versus normal liver. Sp1 overexpression in normal hepatocytes transfected with an Sp1 expression vector induced a marked increased of laminin-gamma1 mRNA content and co-transfection of promoter fragments in Drosophila melanogaster SL2 cells demonstrated that Sp1 transactivates LamC1. In human hepatocellular carcinomas, Sp1 and laminin-gamma1 mRNA were simultaneously expressed at high levels, and gel-shift experiments demonstrated a higher GC-binding activity to Sp1 compared with control livers. In situ hybridization indicated that cells exhibiting a high content of laminin-gamma1 mRNA were also strongly positive for Sp1 mRNA, including both cancer cells at the invasion front and stromal cells. These results show that Sp1 is involved in the activation of LamC1 that occurs in human hepatocellular carcinomas.
Indexed as
Identifiers
9403717PMC1858367W985869684What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.