Evidence map›Paper›PMID 9401972›Full record

ArticleThe Journal of physiology1997

Characterization of the inward current induced by metabotropic glutamate receptor stimulation in rat ventromedial hypothalamic neurones.

K Lee, P R Boden

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of physiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
1.3field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 48 citations in OpenAlex.

  1. Article
  2. Review
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  4. Article
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  8. Article
  9. Circuitry for associative plasticity in the amygdala involves endocannabinoid signaling.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004
    Article
  10. Review
  11. Hypocretin/orexin innervation and excitation of identified septohippocampal cholinergic neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004
    Article
  12. Role of thalamic phospholipase C[beta]4 mediated by metabotropic glutamate receptor type 1 in inflammatory pain.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003
    Article
  13. Orexin excites GABAergic neurons of the arcuate nucleus by activating the sodium--calcium exchanger.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003
    Article
  14. Orexin/hypocretin excites the histaminergic neurons of the tuberomammillary nucleus.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001
    Article
  15. Differential expression of group I metabotropic glutamate receptors in functionally distinct hippocampal interneurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000
    Article
  16. Article
  17. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 2 countries.

K LeeParke-Davis Neuroscience Research Centre, Cambridge, UK. kevin.lee@camb.wl.com
P R Boden
University of Cambridge · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

1. Whole-cell patch clamp recordings were made from rat ventromedial hypothalamic neurones in slices of brain tissue in vitro. Bath application of 50 microM (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid (1S,3R-ACPD) depolarized all neurones tested by activation of an inward current of approximately 55 pA at -60 mV. 2. The inward current elicited by 1S,3R-ACPD was unaffected by K+ channel blockade with external Cs+, Ba2+ or TEA. However, the current was significantly reduced by replacement of the external NaCl with either Tris-HCl or LiCl. 3. The 1S,3R-ACPD-induced current was reduced by the heavy metal ions Ni2+ or La3+ and also by the Na(+)-Ca2+ exchange current inhibitor 3',4'-dichlorobenzamil. 4. The effects of 1S,3R-ACPD were mimicked by the group I metabotropic agonist 3,5-dihydroxyphenylglycine (DHPG) but not by the group III selective agonist, L-2-amino-4-phosphonobutanoate (L-AP4). Furthermore, the effects of 1S,3R-ACPD were inhibited by the metabotropic antagonists alpha-methyl-4-carboxyphenylglycine (MCPG) and 1-aminoindan-1,5-dicarboxylic acid (AIDA) but not by the presynaptic metabotropic receptor antagonists alpha-methyl-4-phosphonophenylglycine (MPPG) or alpha-methyl-4-tetrazolylphenylglycine (MTPG). 5. Photorelease of caged GDP beta S inside neurones irreversibly blocked the 1S,3R-ACPD-induced current whilst photolysis of caged GTP gamma S inside neurones irreversibly potentiated this current. 6. The PLC inhibitor U-73,122 significantly reduced the size of the inward current induced by 1S,3R-ACPD. This effect was not mimicked by the inactive analogue U-73,343. 7. Flash photolysis of the caged calcium chelator diazo-2 inside neurones diminished the response to 1S,3R-ACPD. 8. It is concluded that group I metabotropic glutamate receptors depolarize neurones in the VMH by activation of a Na(+)-Ca2+ exchange current through a G-protein coupled increase in intracellular Ca2+.

Indexed as

AnimalsCell MembraneCycloleucineElectric StimulationElectrophysiologyIn Vitro TechniquesIon ChannelsMaleMembrane PotentialsNeuronsPatch-Clamp TechniquesPhotolysisRatsRats, Sprague-DawleyReceptors, Metabotropic GlutamateSodium1-amino-1,3-dicarboxycyclopentaneCycloleucineIon ChannelsReceptors, Metabotropic GlutamateSodiumType C Phospholipases

Identifiers

PMID9401972
PMCPMC1159968
OpenAlexW2062580011

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.