ArticleJournal of bacteriology1997
Characterization of the aes gene of Escherichia coli encoding an enzyme with esterase activity.
Article in Journal of bacteriology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 70 citations in OpenAlex.
- A Stereospecific Lactic Acid Exclusion Biosensor for Grass Silage Fed Green Biorefinery.Microbial biotechnology · 2026Article
- Structural basis for negative regulation of the Escherichia coli maltose system.Nature communications · 2023Article
- Bacterial bug-out bags: outer membrane vesicles and their proteins and functions.Journal of microbiology (Seoul, Korea) · 2020Review
- Crystal structures of a natural DNA polymerase that functions as an XNA reverse transcriptase.Nucleic acids research · 2019Article
- An engineered small RNA-mediated genetic switch based on a ribozyme expression platform.Nucleic acids research · 2013Article
- Patterns of evolutionary conservation of essential genes correlate with their compensability.PLoS genetics · 2012Article
- Analysis of the genome of the sexually transmitted insect virus Helicoverpa zea nudivirus 2.Viruses · 2012Article
- Exposure to Glycolytic Carbon Sources Reveals a Novel Layer of Regulation for the MalT Regulon.International journal of microbiology · 2011Article
- aes, the gene encoding the esterase B in Escherichia coli, is a powerful phylogenetic marker of the species.BMC microbiology · 2009Article
- A critical examination of Escherichia coli esterase activity.The Journal of biological chemistry · 2009Article
- A critical process controlled by MalT and OmpR is revealed through synthetic lethality.Journal of bacteriology · 2009Article
- Glucose- and glucokinase-controlled mal gene expression in Escherichia coli.Journal of bacteriology · 2009Article
- Crystallization and preliminary X-ray diffraction analysis of ybfF, a new esterase from Escherichia coli K12.Acta crystallographica. Section F, Structural biology and crystallization communications · 2007Article
- Mlc of Thermus thermophilus: a glucose-specific regulator for a glucose/mannose ABC transporter in the absence of the phosphotransferase system.Journal of bacteriology · 2006Article
- Bacillus subtilis cysteine synthetase is a global regulator of the expression of genes involved in sulfur assimilation.Journal of bacteriology · 2005Article
- Decreasing the level of ethyl acetate in ethanolic fermentation broths of Escherichia coli KO11 by expression of Pseudomonas putida estZ esterase.Applied and environmental microbiology · 2002Article
- The N terminus of the Escherichia coli transcription activator MalT is the domain of interaction with MalY.Journal of bacteriology · 2002Article
- A complex signaling module governs the activity of MalT, the prototype of an emerging transactivator family.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Signal transduction between a membrane-bound transporter, PtsG, and a soluble transcription factor, Mlc, of Escherichia coli.The EMBO journal · 2000Article
- X-ray structure of MalY from Escherichia coli: a pyridoxal 5'-phosphate-dependent enzyme acting as a modulator in mal gene expression.The EMBO journal · 2000Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
malQ mutants of Escherichia coli lacking amylomaltase cannot grow on maltose. They express the maltose system constitutively and are sensitive to maltose when grown on another carbon source. In an attempt to isolate a multicopy suppressor that would result in growth on maltose, we transformed a malQ mutant with a gene bank of E. coli DNA which had been digested with Sau3a and cloned in pBR322. We screened the transformants on MacConkey maltose plates. A colony was isolated that appeared to be resistant to maltose and was pink on these plates, but it was still unable to grow on minimal medium with maltose as the carbon source. The plasmid was isolated, and the gene causing this phenotype was characterized. The deduced amino acid sequence of the encoded protein shows homology to that of lipases and esterases. We termed the gene aes, for acetyl esterase. Extracts of cells harboring plasmid-encoded aes under its own promoter exhibit a fivefold higher capacity to hydrolyze p-nitrophenyl acetate than do extracts of cells of plasmid-free strains. Similarly, strains harboring plasmid-encoded aes are able to grow on triacetyl glycerol (triacetin) whereas the plasmid-free strains are not. The expression of plasmid-encoded aes resulted in strong repression of the maltose transport genes in malT+ strains (10-fold reduction), but not in a malT(Con) strain which is independent of the inducer. Also, overproduction of MalT counteracted the Aes-dependent repression, indicating a direct interaction between MalT and Aes.
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