ArticleJournal of clinical microbiology1997
Comparison of PCR, culture, and histopathology for diagnosis of tuberculous pericarditis.
Article in Journal of clinical microbiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 109 citations in OpenAlex.
- Acute Large Pericardial Effusion With Haemodynamic Compromise Secondary to Undiagnosed Tuberculosis.Cureus · 2024Article
- Tuberculous pericardial disease: a focused update on diagnosis, therapy and prevention of complications.Cardiovascular diagnosis and therapy · 2020Review
- Diagnosis and Management of Tuberculous Pericarditis: What Is New?Current cardiology reports · 2020Review
- The waffle procedure as treatment of a first episode of right heart failure: a case report.European heart journal. Case reports · 2018Article
- Tuberculous pericarditis treated with steroid in a dialysis patient.BMJ case reports · 2017Article
- Evaluation of in-house polymerase chain reaction assay sensitivity, can it be utilized in limited-resources settings?Medical journal of the Islamic Republic of Iran · 2014Article
- Tuberculous pericarditis presenting as massive haemorrhagic pericardial effusion.BMJ case reports · 2012Article
- Article
- A 15-year-old girl with a large pericardial effusion.European journal of pediatrics · 2008Article
- Subxiphoid pericardiostomy in the management of pericardial effusions: case series analysis of 368 patients.Heart (British Cardiac Society) · 2005Article
- Diagnosis of tuberculous aetiology in pericardial effusions.Postgraduate medical journal · 2004Review
- Acute pericardial disease: approach to the aetiologic diagnosis.Heart (British Cardiac Society) · 2004Review
- Video-assisted thoracoscopic pericardial fenestration for tuberculous pericardial effusion.The Japanese journal of thoracic and cardiovascular surgery : official publication of the Japanese Association for Thoracic Surgery = Nihon Kyobu Geka Gakkai zasshi · 2004Article
- Comparison of in house polymerase chain reaction with conventional techniques for the detection of Mycobacterium tuberculosis DNA in granulomatous lymphadenopathy.Journal of clinical pathology · 2000Article
- Treatment of Pericardial Effusion Through Subxiphoid Tube Pericardiostomy and Computerized Tomography- or Echocardiography - Guided Percutaneous Catheter Drainage Methods.Brazilian journal of cardiovascular surgeryArticle
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nucleic acid amplification techniques for the diagnosis of tuberculosis (TB) are rapidly being developed. Scant work, however, has focused on pericardial TB. Using cryopreserved specimens from a prior study of pericarditis, we compared PCR to culture and histopathology for the diagnosis of tuberculous pericarditis in 36 specimens of pericardial fluid and 19 specimens of pericardial tissue from 20 patients. Fluid and tissue were cultured on Lowenstein-Jensen and Middlebrook solid media and in BACTEC radiometric broth. Tissue specimens were stained with hematoxylin-eosin, Ziehl-Neelsen, auramine O, and Kinyoun stains and were examined for granuloma formation and acid-fast bacilli. PCR was performed with both fluid and tissue with IS6110-based primers specific for the Mycobacterium tuberculosis complex by published methods. Sixteen of the 20 patients had tuberculous pericarditis and 4 patients had other diagnoses. TB was correctly diagnosed by culture in 15 (93%) patients, by PCR in 13 (81%) patients, and by histology in 13 of 15 (87%) patients. PCR gave one false-positive result for a patient with Staphylococcus aureus pericarditis. Considering the individual specimens as the unit of analysis, M. tuberculosis was identified by culture in 30 of 43 specimens (70%) from patients with tuberculous pericarditis and by PCR in 14 of 28 specimens (50%) from patients with tuberculous pericarditis (P > 0.15). The sensitivity of PCR was higher with tissue specimens (12 of 15; 80%) than with fluid specimens (2 of 13; 15%; P = 0.002). In conclusion, the overall accuracy of PCR approached the results of conventional methods, although PCR was much faster. Therefore, PCR merits further development in this regard. The sensitivity of PCR with pericardial fluid was poor, and false-positive results with PCR remain a concern.
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