ArticleJournal of clinical microbiology1997
Determination of hepatitis C virus genotypes in the United States by cleavase fragment length polymorphism analysis.
Article in Journal of clinical microbiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed.
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- Polymerase Chain Reaction-Electrospray-Time-of-Flight Mass Spectrometry Versus Culture for Bacterial Detection in Septic Arthritis and Osteoarthritis.Genetic testing and molecular biomarkers · 2016Article
- Comparative Genomic Analyses of the Moraxella catarrhalis Serosensitive and Seroresistant Lineages Demonstrate Their Independent Evolution.Genome biology and evolution · 2016Article
- TSH Receptor Cleavage Into Subunits and Shedding of the A-Subunit; A Molecular and Clinical Perspective.Endocrine reviews · 2016Review
- Withdrawn: TSH Receptor Cleavage Into Subunits and Shedding of the A-Subunit; A Molecular and Clinical Perspective.Endocrine reviews · 2016Review
- Ethanol enhances hepatitis C virus replication through lipid metabolism and elevated NADH/NAD+.The Journal of biological chemistry · 2010Article
- Matrix-assisted laser desorption ionization-time of flight (mass spectrometry) for hepatitis C virus genotyping.Journal of clinical microbiology · 2005Article
- High sensitivity EndoV mutation scanning through real-time ligase proofreading.Nucleic acids research · 2004Article
- Exo-proofreading, a versatile SNP scoring technology.Genome research · 2003Article
- Multiplex Pyrosequencing.Nucleic acids research · 2002Article
- Detection of germline BRCA1 mutations by Multiple-Dye Cleavase Fragment Length Polymorphism (MD-CFLP) method.British journal of cancer · 2001Article
- Secondary structure prediction and structure-specific sequence analysis of single-stranded DNA.Nucleic acids research · 2001Article
- Principles and applications of methods for DNA-based typing of microbial organisms.Journal of clinical microbiology · 1999Review
- Use of base excision sequence scanning for detection of genetic variations in St. Louis encephalitis virus isolates.Journal of clinical microbiology · 1999Article
- Genetic methods for assessing antimicrobial resistance.Antimicrobial agents and chemotherapy · 1999Review
- Algorithmic approach to high-throughput molecular screening for alpha interferon-resistant genotypes in hepatitis C patients.Journal of clinical microbiology · 1998Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe the application of a new DNA-scanning method, which has been termed Cleavase Fragment Length Polymorphism (CFLP; Third Wave Technologies, Inc., Madison, Wis.), for the determination of the genotype of hepatitis C virus (HCV). CFLP analysis results in the generation of structural fingerprints that allow discrimination of different DNA sequences. We analyzed 251-bp cDNA products generated by reverse transcription-PCR of the well-conserved 5'-noncoding region of HCV. We determined the genotypes of 87 samples by DNA sequencing and found isolates representing 98% of the types typically encountered in the United States, i.e., types 1a, 1b, 2a/c, 2b, 3a, and 4. Blinded CFLP analysis of these samples was 100% concordant with DNA sequencing results, such that closely related genotypes yielded patterns with strong familial resemblance whereas more divergent sequences yielded patterns with pronounced dissimilarities. In each case, the aggregate pattern was indicative of genotypic grouping, while finer changes suggested subgenotypic differences. We also assessed the reproducibility of CFLP analysis in HCV genotyping by analyzing three distinct isolates belonging to a single subtype. These three isolates yielded indistinguishable CFLP patterns, as did replicate analysis of a single isolate. This study demonstrates the suitability of this technology for HCV genotyping and suggests that it may provide a low-cost, high-throughput alternative to DNA sequencing or other, more costly or cumbersome genotyping approaches.
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