ArticleJournal of clinical microbiology1997
Identification of pathogenic Leptospira genospecies by continuous monitoring of fluorogenic hybridization probes during rapid-cycle PCR.
Article in Journal of clinical microbiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed.
- Article
- Article
- Identification ofPathogens and global health · 2019Article
- Seroprevalence, frequency of leptospiuria, and associated risk factors in horses in Kansas, Missouri, and Nebraska from 2016-2017.PloS one · 2018Article
- Simultaneous detection of chikungunya virus, dengue virus and human pathogenic Leptospira genomes using a multiplex TaqMan® assay.BMC microbiology · 2017Article
- Identification of cattle, buffaloes and rodents as reservoir animals of Leptospira in the District of Gampaha, Sri Lanka.BMC research notes · 2017Article
- Evidence of leptospirosis in the kidneys and serum of feral swine (Sus scrofa) in the United States.Epidemiology and infection · 2017Article
- Human Leptospirosis on Reunion Island, Indian Ocean: Are Rodents the (Only) Ones to Blame?PLoS neglected tropical diseases · 2016Article
- PCR and culture identification of pathogenic Leptospira spp. from coastal soil in Leyte, Philippines, after a storm surge during Super Typhoon Haiyan (Yolanda).Applied and environmental microbiology · 2014Article
- Development and validation of a real-time PCR for detection of pathogenic leptospira species in clinical materials.PloS one · 2009Article
- Possible linkages between lignite aquifers, pathogenic microbes, and renal pelvic cancer in northwestern Louisiana, USA.Environmental geochemistry and health · 2006Article
- Development of a Multiple-Locus Variable Number of Tandem Repeat Analysis (MLVA) for Leptospira interrogans and its application to Leptospira interrogans serovar Australis isolates from Far North Queensland, Australia.Annals of clinical microbiology and antimicrobials · 2005Article
- Advances in real-time PCR: application to clinical laboratory diagnostics.Advances in clinical chemistry · 2005Review
- Three cases of leptospirosis requiring intensive care.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2004Article
- Real-time Fluorescent PCR Techniques to Study Microbial-Host Interactions.Methods in microbiology · 2004Review
- A quantitative PCR (TaqMan) assay for pathogenic Leptospira spp.BMC infectious diseases · 2002Article
- Monitoring the emergence of hepatitis B virus polymerase gene variants during lamivudine therapy using the LightCycler.Journal of clinical microbiology · 2001Article
- Rapid real time PCR to distinguish between high risk human papillomavirus types 16 and 18.Molecular pathology : MP · 2001Article
- Use of real-time PCR and fluorimetry for rapid detection of rifampin and isoniazid resistance-associated mutations in Mycobacterium tuberculosis.Journal of clinical microbiology · 2000Article
- Use of real-time PCR and fluorimetry to detect lamivudine resistance-associated mutations in hepatitis B virus.Antimicrobial agents and chemotherapy · 1999Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Partial sequences of 23S rRNA gene PCR products from 23 strains of 6 pathogenic Leptospira genospecies and from 8 strains of the saprophytic Leptospira biflexa were determined. Sequence analyses enabled Leptospira genus-specific amplification primers and pathogen-specific fluorogenic adjacent hybridization probes to be designed and synthesized. A PCR protocol was developed in which changes in fluorescence emission resulting from specific annealing of fluorogenic adjacent hybridization probes to the target DNA were continuously monitored. Nine strains of the pathogenic Leptospira genospecies could be differentiated from Leptonema illini, Escherichia coli, and eight strains of Leptospira biflexa. The PCR method was rapid, requiring 18 min for the completion of 45 cycles. It was also simple and flexible, as DNA templates prepared by four different methods, including the simple boiling method, could be used without adverse effects. Two hundred copies of target, equivalent to 100 cells, could be detected.
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