ArticleJournal of clinical microbiology1997
Typing of Pneumocystis carinii f. sp. hominis by single-strand conformation polymorphism of four genomic regions.
Article in Journal of clinical microbiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Genotype distribution ofFrontiers in fungal biology · 2026Article
- Selective expression ofmBio · 2025Article
- Consensus Multilocus Sequence Typing Scheme forJournal of fungi (Basel, Switzerland) · 2020Review
- Outbreak-Causing Fungi: Pneumocystis jirovecii.Mycopathologia · 2020Review
- Comparison of MultiLocus Sequence Typing (MLST) and Microsatellite Length Polymorphism (MLP) forComputational and structural biotechnology journal · 2020Article
- Genetic polymorphisms associated with treatment failure and mortality in pediatric Pneumocystosis.Scientific reports · 2019Article
- Article
- A Molecular Window into the Biology and Epidemiology of Pneumocystis spp.Clinical microbiology reviews · 2018Review
- Article
- Diversity of Pneumocystis jirovecii Across Europe: A Multicentre Observational Study.EBioMedicine · 2017Observational
- Investigating Clinical Issues by Genotyping of Medically Important Fungi: Why and How?Clinical microbiology reviews · 2017Review
- Epidemiological Outbreaks of Pneumocystis jirovecii Pneumonia Are Not Limited to Kidney Transplant Recipients: Genotyping Confirms Common Source of Transmission in a Liver Transplantation Unit.Journal of clinical microbiology · 2016Article
- Article
- Prevalence and genotype distribution of Pneumocystis jirovecii in Cuban infants and toddlers with whooping cough.Journal of clinical microbiology · 2014Article
- Multilocus sequence typing of Pneumocystis jirovecii from clinical samples: how many and which loci should be used?Journal of clinical microbiology · 2013Article
- A Pneumocystis jirovecii pneumonia outbreak in a single kidney-transplant center: role of cytomegalovirus co-infection.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2012Article
- Outbreaks of Pneumocystis pneumonia in 2 renal transplant centers linked to a single strain of Pneumocystis: implications for transmission and virulence.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2012Article
- Restriction fragment length polymorphism typing demonstrates substantial diversity among Pneumocystis jirovecii isolates.The Journal of infectious diseases · 2009Article
- Molecular evidence of nosocomial Pneumocystis jirovecii transmission among 16 patients after kidney transplantation.Journal of clinical microbiology · 2008Article
- Genotypic study of Pneumocystis jirovecii in human immunodeficiency virus-positive patients in Thailand.Journal of clinical microbiology · 2005Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To better investigate Pneumocystis carinii f. sp. hominis epidemiology, we have developed a molecular typing method. Because of the limited genetic variability of the P. carinii hominis genome, a multitarget approach was used. Four variable regions of the genome were amplified by PCR, polymorphism in each region was assessed by the single-strand conformation polymorphism (SSCP) technique, and the results for the four regions of each patient were combined. Bronchoalveolar lavage specimens collected from 11 patients were examined. Four patients were probably infected by a single strain, since their specimens yielded simple SSCP patterns (two bands corresponding to one allele). The combinations of these patterns were unique, suggesting that the strains which infected these patients were different. For the other seven patients, complex patterns were found (three or four bands corresponding to two alleles). The presence of more than one allele of a region in a patient is likely to be due to coinfection. Polymorphism was also assessed by sequencing, which revealed variations at nucleotide positions previously reported to vary. About half of the observed alleles had already been reported by laboratories in different countries. Multitarget typing of P. carinii hominis by PCR-SSCP should allow investigation of strain diversity and thus be useful for future epidemiological studies.
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