ArticleMolecular biology of the cell1997
Myosin heavy chain phosphorylation sites regulate myosin localization during cytokinesis in live cells.
Article in Molecular biology of the cell, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 86 citations in OpenAlex.
- One must reconstitute the functions of interest from purified proteins.Frontiers in physiology · 2024Review
- MYH10 Governs Adipocyte Function and Adipogenesis through Its Interaction with GLUT4.International journal of molecular sciences · 2022Article
- Myosin IIB assembly state determines its mechanosensitive dynamics.The Journal of cell biology · 2019Article
- Structure of the Dictyostelium Myosin-II Heavy Chain Kinase A (MHCK-A) α-kinase domain apoenzyme reveals a novel autoinhibited conformation.Scientific reports · 2016Article
- Cell shape regulation through mechanosensory feedback control.Journal of the Royal Society, Interface · 2015Article
- Genetic suppression of a phosphomimic myosin II identifies system-level factors that promote myosin II cleavage furrow accumulation.Molecular biology of the cell · 2014Article
- Cytokinesis mechanics and mechanosensing.Cytoskeleton (Hoboken, N.J.) · 2012Review
- Molecular motors: forty years of interdisciplinary research.Molecular biology of the cell · 2011Article
- Stretching actin filaments within cells enhances their affinity for the myosin II motor domain.PloS one · 2011Article
- Biphasic targeting and cleavage furrow ingression directed by the tail of a myosin II.The Journal of cell biology · 2010Article
- 14-3-3, an integrator of cell mechanics and cytokinesis.Small GTPases · 2010Article
- Determinants of myosin II cortical localization during cytokinesis.Current biology : CB · 2010Article
- Mechanosensing through cooperative interactions between myosin II and the actin crosslinker cortexillin I.Current biology : CB · 2009Article
- Identification and characterization of Myosin from rat testicular peritubular myoid cells.Biology of reproduction · 2008Article
- Article
- A global, myosin light chain kinase-dependent increase in myosin II contractility accompanies the metaphase-anaphase transition in sea urchin eggs.Molecular biology of the cell · 2006Article
- Characterization of the minimum domain required for targeting budding yeast myosin II to the site of cell division.BMC biology · 2006Article
- Multiple myosin II heavy chain kinases: roles in filament assembly control and proper cytokinesis in Dictyostelium.Molecular biology of the cell · 2005Article
- A mitotic kinesin-like protein required for normal karyokinesis, myosin localization to the furrow, and cytokinesis in Dictyostelium.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Dictyostelium myosin bipolar thick filament formation: importance of charge and specific domains of the myosin rod.PLoS biology · 2004Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Conventional myosin II plays a fundamental role in the process of cytokinesis where, in the form of bipolar thick filaments, it is thought to be the molecular motor that generates the force necessary to divide the cell. In Dictyostelium, the formation of thick filaments is regulated by the phosphorylation of three threonine residues in the tail region of the myosin heavy chain. We report here on the effects of this regulation on the localization of myosin in live cells undergoing cytokinesis. We imaged fusion proteins of the green-fluorescent protein with wild-type myosin and with myosins where the three critical threonines had been changed to either alanine or aspartic acid. We provide evidence that thick filament formation is required for the accumulation of myosin in the cleavage furrow and that if thick filaments are overproduced, this accumulation is markedly enhanced. This suggests that myosin localization in dividing cells is regulated by myosin heavy chain phosphorylation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.