ArticleMolecular biology of the cell1997
Characterization of Saccharomyces cerevisiae dna2 mutants suggests a role for the helicase late in S phase.
Article in Molecular biology of the cell, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 57 citations in OpenAlex.
- Review
- The secondary-structured DNA-binding activity of Dna2 endonuclease/helicase is critical to cell growth under replication stress.The FEBS journal · 2021Article
- Multiple roles of DNA2 nuclease/helicase in DNA metabolism, genome stability and human diseases.Nucleic acids research · 2020Review
- Sumoylation regulates the stability and nuclease activity ofCommunications biology · 2019Article
- Article
- Replication intermediates that escape Dna2 activity are processed by Holliday junction resolvase Yen1.Nature communications · 2016Article
- The Saccharomyces cerevisiae Dna2 can function as a sole nuclease in the processing of Okazaki fragments in DNA replication.Nucleic acids research · 2015Article
- Rad52/Rad59-dependent recombination as a means to rectify faulty Okazaki fragment processing.The Journal of biological chemistry · 2014Article
- Okazaki fragment processing-independent role for human Dna2 enzyme during DNA replication.The Journal of biological chemistry · 2012Article
- Inviability of a DNA2 deletion mutant is due to the DNA damage checkpoint.Cell cycle (Georgetown, Tex.) · 2011Article
- Article
- An iron-sulfur cluster is essential for the binding of broken DNA by AddAB-type helicase-nucleases.The Journal of biological chemistry · 2009Article
- Interplay of Mre11 nuclease with Dna2 plus Sgs1 in Rad51-dependent recombinational repair.PloS one · 2009Article
- A network of multi-tasking proteins at the DNA replication fork preserves genome stability.PLoS genetics · 2005Article
- Enzymatic properties of the Caenorhabditis elegans Dna2 endonuclease/helicase and a species-specific interaction between RPA and Dna2.Nucleic acids research · 2005Article
- Genetics of lagging strand DNA synthesis and maturation in fission yeast: suppression analysis links the Dna2-Cdc24 complex to DNA polymerase delta.Nucleic acids research · 2004Article
- The human Bloom syndrome gene suppresses the DNA replication and repair defects of yeast dna2 mutants.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- LST8 negatively regulates amino acid biosynthesis as a component of the TOR pathway.The Journal of cell biology · 2003Article
- Dynamic localization of an Okazaki fragment processing protein suggests a novel role in telomere replication.Molecular and cellular biology · 2002Article
- Mutations in DNA replication genes reduce yeast life span.Molecular and cellular biology · 2002Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The TOR proteins, originally identified as targets of the immunosuppressant rapamycin, contain an ATM-like "lipid kinase" domain and are required for early G1 progression in eukaryotes. Using a screen to identify Saccharomyces cerevisiae mutants requiring overexpression of Tor1p for viability, we have isolated mutations in a gene we call ROT1 (requires overexpression of Tor1p). This gene is identical to DNA2, encoding a helicase required for DNA replication. As with its role in cell cycle progression, both the N-terminal and C-terminal regions, as well as the kinase domain of Tor1p, are required for rescue of dna2 mutants. Dna2 mutants are also rescued by Tor2p and show synthetic lethality with tor1 deletion mutants under specific conditions. Temperature-sensitive (Ts) dna2 mutants arrest irreversibly at G2/M in a RAD9- and MEC1-dependent manner, suggesting that Dna2p has a role in S phase. Frequencies of mitotic recombination and chromosome loss are elevated in dna2 mutants, also supporting a role for the protein in DNA synthesis. Temperature-shift experiments indicate that Dna2p functions during late S phase, although dna2 mutants are not deficient in bulk DNA synthesis. These data suggest that Dna2p is not required for replication fork progression but may be needed for a later event such as Okazaki fragment maturation.
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