ArticleMolecular biology of the cell1997
Inhibition of RhoA translocation and calcium sensitization by in vivo ADP-ribosylation with the chimeric toxin DC3B.
Article in Molecular biology of the cell, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed, 151 citations in OpenAlex.
- Loss of NUMB drives aggressive bladder cancer via a RHOA/ROCK/YAP signaling axis.Nature communications · 2024Article
- A Novel Single-Color FRET Sensor for Rho-Kinase Reveals Calcium-Dependent Activation of RhoA and ROCK.Sensors (Basel, Switzerland) · 2024Article
- Calcium signaling cascades differentially regulate PGFNaunyn-Schmiedeberg's archives of pharmacology · 2021Article
- Calcium desensitisation in late polymicrobial sepsis is associated with loss of vasopressor sensitivity in a murine model.Intensive care medicine experimental · 2015Article
- Attenuated platelet aggregation in patients with septic shock is independent from the activity state of myosin light chain phosphorylation or a reduction in Rho kinase-dependent inhibition of myosin light chain phosphatase.Intensive care medicine experimental · 2015Article
- Clostridial C3 Toxins Target Monocytes/Macrophages and Modulate Their Functions.Frontiers in immunology · 2015Review
- Viral activation of MK2-hsp27-p115RhoGEF-RhoA signaling axis causes cytoskeletal rearrangements, p-body disruption and ARE-mRNA stabilization.PLoS pathogens · 2015Article
- Bacterial factors exploit eukaryotic Rho GTPase signaling cascades to promote invasion and proliferation within their host.Small GTPases · 2014Review
- Augmented bronchial smooth muscle contractility induced by aqueous cigarette smoke extract in rats.Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi · 2014Article
- Soluble guanylate cyclase generation of cGMP regulates migration of MGE neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Direct binding and regulation of RhoA protein by cyclic GMP-dependent protein kinase Iα.The Journal of biological chemistry · 2012Article
- RhoA/ROCK pathway is the major molecular determinant of basal tone in intact human internal anal sphincter.American journal of physiology. Gastrointestinal and liver physiology · 2012Article
- Article
- Therapeutic effects of Clostridium botulinum C3 exoenzyme.Naunyn-Schmiedeberg's archives of pharmacology · 2011Review
- The Staphylococcus aureus epidermal cell differentiation inhibitor toxin promotes formation of infection foci in a mouse model of bacteremia.Infection and immunity · 2010Article
- The effects of the small GTPase RhoA on the muscarinic contraction of airway smooth muscle result from its role in regulating actin polymerization.American journal of physiology. Cell physiology · 2010Article
- 3-Hydroxymethyl coenzyme A reductase inhibition attenuates spontaneous smooth muscle tone via RhoA/ROCK pathway regulated by RhoA prenylation.American journal of physiology. Gastrointestinal and liver physiology · 2010Article
- RhoA/Rho-kinase: pathophysiologic and therapeutic implications in gastrointestinal smooth muscle tone and relaxation.Gastroenterology · 2010Review
- Inhibition of myosin light chain phosphorylation decreases rat mesenteric lymphatic contractile activity.American journal of physiology. Heart and circulatory physiology · 2009Article
- C3 exoenzymes, novel insights into structure and action of Rho-ADP-ribosylating toxins.Naunyn-Schmiedeberg's archives of pharmacology · 2007Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Pretreatment of intact rabbit portal vein smooth muscle with the chimeric toxin DC3B (10(-6) M, 48 h; ; ) ADP-ribosylated endogenous RhoA, including cytosolic RhoA complexed with rhoGDI, and inhibited the tonic phase of phenylephrine-induced contraction and the Ca2+-sensitization of force by phenylephrine, endothelin and guanosine triphosphate (GTP)gammaS, but did not inhibit Ca2+-sensitization by phorbol dibutyrate. DC3B also inhibited GTPgammaS-induced translocation of cytosolic RhoA () to the membrane fraction. In DC3B-treated muscles the small fraction of membrane-associated RhoA could be immunoprecipitated, even after exposure to GTPgammaS, which prevents immunoprecipitation of non-ADP-ribosylated RhoA. Dissociation of cytosolic RhoA-rhoGDI complexes with SDS restored the immunoprecipitability and ADP ribosylatability of RhoA, indicating that both the ADP-ribosylation site (Asn 41) and RhoA insert loop (Wei et al., 1997) are masked by rhoGDI and that the long axes of the two proteins are in parallel in the heterodimer. We conclude that RhoA plays a significant role in G-protein-, but not protein kinase C-mediated, Ca2+ sensitization and that ADP ribosylation inhibits in vivo the Ca2+-sensitizing effect of RhoA by interfering with its binding to a membrane-associated effector.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.