ArticleThe Journal of cell biology1997
Compartmentalized IgE receptor-mediated signal transduction in living cells.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
62 citing papers in PubMed, 187 citations in OpenAlex.
- Critical roles of plasma membrane dynamics and heterogeneity at the early stage of antigen-stimulated FcεRI signaling in mast cells.Biochemical Society transactions · 2025Review
- Design and evaluation of engineered protein biosensors for live-cell imaging of EGFR phosphorylation.Science signaling · 2019Article
- Dimerization of glycoprotein Ibα is not sufficient to induce platelet clearance.Journal of thrombosis and haemostasis : JTH · 2016Article
- Sphingolipid Organization in the Plasma Membrane and the Mechanisms That Influence It.Frontiers in cell and developmental biology · 2016Review
- An Interaction Library for the FcεRI Signaling Network.Frontiers in immunology · 2014Review
- Molecular mechanisms of spontaneous and directed mast cell motility.Journal of leukocyte biology · 2012Article
- Non-genomic inhibitory effect of glucocorticoids on activated peripheral blood basophils through suppression of lipid raft formation.Clinical and experimental immunology · 2012Article
- Live-cell fluorescence microscopy with molecular biosensors: what are we really measuring?Biophysical journal · 2012Article
- Article
- Particulate allergens potentiate allergic asthma in mice through sustained IgE-mediated mast cell activation.The Journal of clinical investigation · 2011Article
- Spatio-temporal signaling in mast cells.Advances in experimental medicine and biology · 2011Review
- Lipid rafts in mast cell biology.Journal of lipids · 2011Article
- Effects of beta-cyclodextrin on the structure of sphingomyelin/cholesterol model membranes.Biophysical journal · 2010Article
- Regulatory activity of polyunsaturated fatty acids in T-cell signaling.Progress in lipid research · 2010Review
- Down-regulation of protein-tyrosine phosphatases activates an immune receptor in the absence of its translocation into lipid rafts.The Journal of biological chemistry · 2010Article
- Formation of a mast cell synapse: Fc epsilon RI membrane dynamics upon binding mobile or immobilized ligands on surfaces.Journal of immunology (Baltimore, Md. : 1950) · 2010Article
- Supported bilayers at the vanguard of immune cell activation studies.Journal of structural biology · 2009Review
- Theory of raft formation by the cross-linking of saturated or unsaturated lipids in model lipid bilayers.Biophysical journal · 2009Article
- Real-time cross-correlation image analysis of early events in IgE receptor signaling.Biophysical journal · 2008Article
- Persistence of cooperatively stabilized signaling clusters drives T-cell activation.Molecular and cellular biology · 2006Article
2 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Several receptor-mediated signal transduction pathways, including EGF and IgE receptor pathways, have been proposed to be spatially restricted to plasma membrane microdomains. However, the experimental evidence for signaling events in these microdomains is largely based on biochemical fractionation and immunocytochemical studies and only little is known about their spatial dynamics in living cells. Here we constructed green fluorescent protein-tagged SH2 domains to investigate where and when IgE receptor (FcepsilonRI)-mediated tyrosine phosphorylation occurs in living tumor mast cells. Strikingly, within minutes after antigen addition, tandem SH2 domains from Syk or PLC-gamma1 translocated from a uniform cytosolic distribution to punctuate plasma membrane microdomains. Colocalization experiments showed that the microdomains where tyrosine phosphorylation occurred were indistinguishable from those stained by cholera toxin B, a marker for glycosphingolipids. Competitive binding studies with coelectroporated unlabeled Syk, PLC-gamma1, and other SH2 domains selectively suppressed the induction of IgE receptor-mediated calcium signals as well as the binding of the fluorescent SH2 domains. This supports the hypothesis that PLC-gamma1 and Syk SH2 domains selectively bind to Syk and IgE receptors, respectively. Unlike the predicted prelocalization of EGF receptors to caveolae microdomains, fluorescently labeled IgE receptors were found to be uniformly distributed in the plasma membrane of unstimulated cells and only transiently translocated to glycosphingolipid rich microdomains after antigen addition. Thus, these in vivo studies support a plasma membrane signaling mechanism by which IgE receptors transiently associate with microdomains and induce the spatially restricted activation of Syk and PLC-gamma1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.