ArticleThe Journal of cell biology1997
Peroxisomal targeting, import, and assembly of alcohol oxidase in Pichia pastoris.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Evolution of alcohol oxidase for improved methanol bioconversion and formaldehyde tolerance.Synthetic and systems biotechnology · 2027Article
- Systematic metabolic engineering of Komagataella phaffii for methanol-to-lactate bioconversion.Bioprocess and biosystems engineering · 2026Article
- Sequence and taxonomic feature evaluation facilitated the discovery of alcohol oxidases.Synthetic and systems biotechnology · 2025Article
- Impact of Oxygen Availability on the Organelle-Specific Redox Potentials and Stress in Recombinant Protein Producing Komagataella phaffii.Microbial biotechnology · 2025Article
- Thermotolerant yeasts promoting climate-resilient bioproduction.FEMS yeast research · 2025Review
- Challenges in elucidating ethylene glycol metabolism in Saccharomyces cerevisiae.FEMS yeast research · 2025Article
- Engineering Yeast Peroxisomes for α-Bisabolene Production from Sole Methanol with the Aid of Proteomic Analysis.JACS Au · 2024Article
- Design and Construction of Artificial Biological Systems for One-Carbon Utilization.Biodesign research · 2023Review
- Use of transcriptomic profiling to identify candidate genes involved in Polyporus umbellatus sclerotial formation affected by oxalic acid.Scientific reports · 2021Article
- Peroxisome Maintenance Depends on De Novo Peroxisome Formation in Yeast Mutants Defective in Peroxisome Fission and Inheritance.International journal of molecular sciences · 2019Article
- Structure-Based Engineering of Phanerochaete chrysosporium Alcohol Oxidase for Enhanced Oxidative Power toward Glycerol.Biochemistry · 2018Article
- Pnc1 piggy-back import into peroxisomes relies on Gpd1 homodimerisation.Scientific reports · 2017Article
- Crystal Structure of Alcohol Oxidase from Pichia pastoris.PloS one · 2016Article
- Systems-level organization of yeast methylotrophic lifestyle.BMC biology · 2015Article
- Multiple pathways for protein transport to peroxisomes.Journal of molecular biology · 2015Review
- Rational design of a fibroblast growth factor 21-based clinical candidate, LY2405319.PloS one · 2013Article
- Protein quaternary structure and expression levels contribute to peroxisomal-targeting-sequence-1-mediated peroxisomal import of human soluble epoxide hydrolase.Journal of molecular biology · 2008Article
- Characteristics of Gloeophyllum trabeum alcohol oxidase, an extracellular source of H2O2 in brown rot decay of wood.Applied and environmental microbiology · 2007Article
- Routing of Hansenula polymorpha alcohol oxidase: an alternative peroxisomal protein-sorting machinery.Molecular biology of the cell · 2004Article
- Contribution of the dimeric state to the thermal stability of the flavoprotein D-amino acid oxidase.Protein science : a publication of the Protein Society · 2003Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Alcohol oxidase (AOX), the first enzyme in the yeast methanol utilization pathway is a homooctameric peroxisomal matrix protein. In peroxisome biogenesis-defective (pex) mutants of the yeast Pichia pastoris, AOX fails to assemble into active octamers and instead forms inactive cytoplasmic aggregates. The apparent inability of AOX to assemble in the cytoplasm contrasts with other peroxisomal proteins that are able to oligomerize before import. To further investigate the import of AOX, we first identified its peroxisomal targeting signal (PTS). We found that sequences essential for targeting AOX are primarily located within the four COOH-terminal amino acids of the protein leucine-alanine-arginine-phenylalanine COOH (LARF). To examine whether AOX can oligomerize before import, we coexpressed AOX without its PTS along with wild-type AOX and determined whether the mutant AOX could be coimported into peroxisomes. To identify the mutant form of AOX, the COOH-terminal LARF sequence of the protein was replaced with a hemagglutinin epitope tag (AOX-HA). Coexpression of AOX-HA with wild-type AOX (AOX-WT) did not result in an increase in the proportion of AOX-HA present in octameric active AOX, suggesting that newly synthesized AOX-HA cannot oligomerize with AOX-WT in the cytoplasm. Thus, AOX cannot initiate oligomerization in the cytoplasm, but must first be targeted to the organelle before assembly begins.
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