ArticleThe Journal of cell biology1997
Probing the architecture of a simple kinetochore using DNA-protein crosslinking.
Article in The Journal of cell biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
52 citing papers in PubMed.
- Native yeast kinetochore structures identify an essential inner kinetochore interaction.Nature communications · 2026Article
- Native yeast kinetochore structures identify an essential inner kinetochore interaction.bioRxiv : the preprint server for biology · 2026Article
- Structure of the Centromere Binding Factor 3 Complex from Kluyveromyces lactis.Journal of molecular biology · 2019Article
- Architecture of the CBF3-centromere complex of the budding yeast kinetochore.Nature structural & molecular biology · 2018Article
- Insights into Centromere DNA Bending Revealed by the Cryo-EM Structure of the Core Centromere Binding Factor 3 with Ndc10.Cell reports · 2018Article
- Structural basis for assembly of the CBF3 kinetochore complex.The EMBO journal · 2018Article
- A Molecular View of Kinetochore Assembly and Function.Biology · 2017Review
- Review
- Structure of yeast kinetochore Ndc10 DNA-binding domain reveals unexpected evolutionary relationship to tyrosine recombinases.The Journal of biological chemistry · 2012Article
- Ndc10 is a platform for inner kinetochore assembly in budding yeast.Nature structural & molecular biology · 2011Article
- Endogenous transcription at the centromere facilitates centromere activity in budding yeast.Current biology : CB · 2011Article
- The centromeric nucleosome of budding yeast is perfectly positioned and covers the entire centromere.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Scm3 is a centromeric nucleosome assembly factor.The Journal of biological chemistry · 2011Article
- Psh1 is an E3 ubiquitin ligase that targets the centromeric histone variant Cse4.Molecular cell · 2010Article
- Hsp90-Sgt1 and Skp1 target human Mis12 complexes to ensure efficient formation of kinetochore-microtubule binding sites.The Journal of cell biology · 2010Article
- Molecular structures and interactions in the yeast kinetochore.Cold Spring Harbor symposia on quantitative biology · 2010Article
- Cse4 is part of an octameric nucleosome in budding yeast.Molecular cell · 2009Article
- In vivo protein architecture of the eukaryotic kinetochore with nanometer scale accuracy.Current biology : CB · 2009Article
- Fission yeast Scm3 mediates stable assembly of Cnp1/CENP-A into centromeric chromatin.Molecular cell · 2009Article
- Structural and functional dissection of Mif2p, a conserved DNA-binding kinetochore protein.Molecular biology of the cell · 2008Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
In budding yeast, accurate chromosome segregation requires that one and only one kinetochore assemble per chromosome. In this paper, we report the use of DNA-protein crosslinking and nondenaturing gel analysis to study the structure of CBF3, a four-protein complex that binds to the essential CDEIII region of Saccharomyces cerevisiae centromeres. We find that three subunits of CBF3 are in direct contact with CDEIII over a region of DNA that spans 80 bp. A highly asymmetric core complex containing p58(CTF13) p64(CEP3) and p110(NDC10) in direct contact with DNA forms at the genetically defined center of CDEIII. This core complex spans approximately 56 bp of CEN3. An extended complex comprising the core complex and additional DNA-bound p110(NDC10) also forms. It spans approximately 80 bp of DNA. CBF3 makes sequence-specific and -nonspecific contacts with DNA. Both contribute significantly to the energy of CBF3-DNA interaction. Moreover, important sequence-specific contacts are made with bases that are not conserved among yeast centromeres. These findings provide a foundation for understanding the organization of the CBF3-centromere complex, a structure that appears to initiate the formation of microtubule attachment sites at yeast kinetochores. These results also have implications for understanding centromere-binding proteins in higher cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.