ArticleThe Biochemical journal1997
Alternative mRNA splicing of 3'-terminal exons generates ascorbate peroxidase isoenzymes in spinach (Spinacia oleracea) chloroplasts.
Article in The Biochemical journal, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 61 citations in OpenAlex.
- Ascorbate peroxidase in fruits and modulation of its activity by reactive species.Journal of experimental botany · 2024Review
- An oligonucleotide/oligosaccharide-binding-fold protein enhances the alternative splicing event producing thylakoid membrane-bound ascorbate peroxidase in Nicotiana tabacum.G3 (Bethesda, Md.) · 2022Article
- Alternative Splicing and Its Roles in Plant Metabolism.International journal of molecular sciences · 2022Review
- Dissecting the Molecular Regulation of Natural Variation in Growth and Senescence of TwoInternational journal of molecular sciences · 2022Article
- Ascorbate Peroxidase Neofunctionalization at the Origin of APX-R and APX-L: Evidence from Basal Archaeplastida.Antioxidants (Basel, Switzerland) · 2021Article
- Catalase and ascorbate peroxidase-representative H2O2-detoxifying heme enzymes in plants.Environmental science and pollution research international · 2016Review
- Genome-wide determination of poly(A) sites in Medicago truncatula: evolutionary conservation of alternative poly(A) site choice.BMC genomics · 2014Article
- Influence of heat stress on leaf ultrastructure, photosynthetic performance, and ascorbate peroxidase gene expression of two pear cultivars (Pyrus pyrifolia).Journal of Zhejiang University. Science. B · 2013Article
- Antioxidant Defenses in Plants with Attention to Prunus and Citrus spp.Antioxidants (Basel, Switzerland) · 2013Review
- Physiological and proteomic responses of diploid and tetraploid black locust (Robinia pseudoacacia L.) subjected to salt stress.International journal of molecular sciences · 2013Article
- Comparative proteomics analysis of proteins expressed in the I-1 and I-2 internodes of strawberry stolons.Proteome science · 2011Article
- Analysis of the molecular evolutionary history of the ascorbate peroxidase gene family: inferences from the rice genome.Journal of molecular evolution · 2004Article
- Thylakoid-bound ascorbate peroxidase mutant exhibits impaired electron transport and photosynthetic activity.Plant physiology · 2003Article
- Cloning and sequencing of cDNAs for hypothetical genes from chromosome 2 of Arabidopsis.Plant physiology · 2002Article
- The water-water cycle as alternative photon and electron sinks.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2000Review
- Patterns of variant polyadenylation signal usage in human genes.Genome research · 2000Article
- Expression of spinach ascorbate peroxidase isoenzymes in response to oxidative stresses.Plant physiology · 2000Article
- Alternatively spliced mRNA variants of chloroplast ascorbate peroxidase isoenzymes in spinach leaves.The Biochemical journal · 1999Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have isolated two cDNA clones encoding spinach (Spinacia oleracea) stromal and thylakoid-bound ascorbate peroxidase isoenzymes [Ishikawa, Sakai, Yoshimura, Takeda and Shigeoka (1996) FEBS Lett. 384, 289-293]. The gene (ApxII) encoding both chloroplastic ascorbate peroxidase isoenzymes was isolated and the organization of the gene was determined. Alignment between the cDNAs and the gene for chloroplastic ascorbate peroxidase isoenzymes indicates that both enzymes arise from a common pre-mRNA by alternative splicing of two 3'-terminal exons. Genomic Southern-blot analysis supported this finding. The gene spanned nearly 8.5 kbp and contained 13 exons split by 12 introns. The penultimate exon 12 (residues 7376-7530) for the stromal ascorbate peroxidase mRNA consisted of one codon for Asp365 before the TAA termination codon, and the entire 3'-untranslated region, including a potential polyadenylation signal (AATAAA). The final exon 13 (residues 7545-7756) for the thylakoid-bound ascorbate peroxidase mRNA consisted of the corresponding coding sequence of the hydrophobic C-terminal region, the TGA termination codon and the entire 3'-untranslated region, including a potential polyadenylation signal (AATATA). Both exons were interrupted by a 14 bp non-coding sequence. Northern-blot and reverse transcription-PCR analysis showed that the transcripts for stromal and thylakoid-bound ascorbate peroxidase are present in spinach leaves.
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