ArticleJournal of bacteriology1997
A gene (wbbL) from Serratia marcescens N28b (O4) complements the rfb-50 mutation of Escherichia coli K-12 derivatives.
Article in Journal of bacteriology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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67 citing papers in PubMed.
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- Mapping Structural Constraints and Adaptive Potential in a Capsule-Degrading Phage Tailspike Protein.bioRxiv : the preprint server for biology · 2025Article
- Two receptor-targeting mechanisms of lambda-like siphophage Gifsy-1 of Salmonella Typhimurium.PLoS pathogens · 2025Article
- Roles of SPI-2 T3SS effectors in virulence ofFrontiers in veterinary science · 2025Article
- A role for the Gram-negative outer membrane in bacterial shape determination.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Preference of Bacterial Rhamnosyltransferases for 6-Deoxysugars Reveals a Strategy To Deplete O-Antigens.Journal of the American Chemical Society · 2023Article
- A role for the Gram-negative outer membrane in bacterial shape determination.bioRxiv : the preprint server for biology · 2023Article
- Immunogenicity and protection of a Pasteurella multocida strain with a truncated lipopolysaccharide outer core in ducks.Veterinary research · 2022Article
- Distinct heterochromatin-like domains promote transcriptional memory and silence parasitic genetic elements in bacteria.The EMBO journal · 2022Article
- Resistance-Nodulation-Cell Division (RND) Transporter AcrD Confers Resistance to Egg White inFoods (Basel, Switzerland) · 2021Article
- The lipopolysaccharide outer core transferase genes pcgD and hptE contribute differently to the virulence of Pasteurella multocida in ducks.Veterinary research · 2021Article
- A T3SS Regulator Mutant ofFrontiers in cellular and infection microbiology · 2020Article
- Polar Flagella Glycosylation inFrontiers in microbiology · 2020Article
- Group A, B, C, and GThe Journal of biological chemistry · 2019Article
- Comparative Genomic Analysis and Characterization of TwoFrontiers in microbiology · 2018Article
- Role of theIndian journal of microbiology · 2017Article
- Two NovelFrontiers in cellular and infection microbiology · 2017Article
7 more citing papers are in PubMed but not listed here.
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8 authors.
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Abstract
A cosmid-based genomic library of Serratia marcescens N28b was introduced into Escherichia coli DH5alpha, and clones were screened for serum resistance. One clone was found resistant to serum, to bacteriocin 28b, and to bacteriophages TuIa and TuIb. This clone also showed O antigen in its lipopolysaccharide. Subcloning and sequencing experiments showed that a 2,124-bp DNA fragment containing the rmlD and wbbL genes was responsible for the observed phenotypes. On the basis of amino acid similarity, we suggest that the 288-residue RmlD protein is a dTDP-L-rhamnose synthase. Plasmid pJT102, containing only the wbbL gene, was able to induce O16-antigen production and serum resistance in E. coli DH5alpha. These results suggest that the 282-residue WbbL protein is a rhamnosyltransferase able to complement the rJb-50 mutation in E. coli K-12 derivatives, despite the low level of amino acid identity between WbbL and the E. coli rhamnosyltransferase (24.80%). S. marcescens N28b rmlD and wbbL mutants were constructed by mobilization of suicide plasmids containing a portion of rmlD or wbbL. These insertion mutants were unable to produce O antigen; since strain N28b produces O4 antigen, these results suggest that both genes are involved in O4-antigen biosynthesis.
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