ArticleJournal of bacteriology1997
A study of iterative type II polyketide synthases, using bacterial genes cloned from soil DNA: a means to access and use genes from uncultured microorganisms.
Article in Journal of bacteriology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
48 citing papers in PubMed, 150 citations in OpenAlex.
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- Genomic and functional techniques to mine the microbiome for novel antimicrobials and antimicrobial resistance genes.Annals of the New York Academy of Sciences · 2017Review
- Culture-independent discovery of natural products from soil metagenomes.Journal of industrial microbiology & biotechnology · 2016Review
- Acylation of Biomolecules in Prokaryotes: a Widespread Strategy for the Control of Biological Function and Metabolic Stress.Microbiology and molecular biology reviews : MMBR · 2015Review
- Mining soil metagenomes to better understand the evolution of natural product structural diversity: pentangular polyphenols as a case study.Journal of the American Chemical Society · 2014Article
- Mining the metabiome: identifying novel natural products from microbial communities.Chemistry & biology · 2014Review
- Arixanthomycins A-C: Phylogeny-guided discovery of biologically active eDNA-derived pentangular polyphenols.ACS chemical biology · 2014Article
- Functional analysis of environmental DNA-derived microviridins provides new insights into the diversity of the tricyclic peptide family.Applied and environmental microbiology · 2014Article
- Arimetamycin A: improving clinically relevant families of natural products through sequence-guided screening of soil metagenomes.Angewandte Chemie (International ed. in English) · 2013Article
- Platforms for antibiotic discovery.Nature reviews. Drug discovery · 2013Review
- Acetoacetyl-CoA synthetase activity is controlled by a protein acetyltransferase with unique domain organization in Streptomyces lividans.Molecular microbiology · 2013Article
- Metagenomic analysis of Streptomyces lividans reveals host-dependent functional expression.Applied and environmental microbiology · 2012Article
- Insight into the molecular basis of aromatic polyketide cyclization: crystal structure and in vitro characterization of WhiE-ORFVI.Biochemistry · 2012Article
- Strategies for discovery and improvement of enzyme function: state of the art and opportunities.Microbial biotechnology · 2012Review
- Functional analysis of environmental DNA-derived type II polyketide synthases reveals structurally diverse secondary metabolites.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
To examine as randomly as possible the role of the beta-ketoacyl and acyl carrier protein (ACP) components of bacterial type II polyketide synthases (PKSs), homologs of the chain-length-factor (CLF) genes were cloned from the environmental community of microorganisms. With PCR primers derived from conserved regions of known ketosynthase (KSalpha) and ACP genes specifying the formation of 16- to 24-carbon polyketides, two CLF (KSbeta) genes were cloned from unclassified streptomycetes isolated from the soil, and two were cloned from soil DNA without the prior isolation of the parent microorganism. The sequence and deduced product of each gene were distinct from those of known KSbeta genes and, by phylogenetic analysis, belonged to antibiotic-producing PKS gene clusters. Hybrid PKS gene cassettes were constructed with each novel KSbeta gene substituted for the actI-ORF2 or tcmL KSbeta subunit genes, along with the respective actI-ORF1 or tcmK KSalpha, tcmM ACP, and tcmN cyclase genes, and were found to produce an octaketide or decaketide product characteristic of the ones known to be made by the heterologous KSalpha gene partner. Since substantially less than 1% of the microorganisms present in soil are thought to be cultivatable by standard methods, this work demonstrates a potential way to gain access to a more extensive range of microbial molecular diversity and to biosynthetic pathways whose products can be tested for biological applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.