ArticleProceedings of the National Academy of Sciences of the United States of America1997
The seven-transmembrane spanning topography of the Alzheimer disease-related presenilin proteins in the plasma membranes of cultured cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 55 citations in OpenAlex.
- Structural investigation of the C-terminal catalytic fragment of presenilin 1.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Towards genome-scale structure prediction for transmembrane proteins.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2006Review
- An early specific cell-cell interaction occurs in the production of beta-amyloid in cell cultures.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Evidence that the COOH terminus of human presenilin 1 is located in extracytoplasmic space.American journal of physiology. Cell physiology · 2005Article
- The Caenorhabditis elegans IMPAS gene, imp-2, is essential for development and is functionally distinct from related presenilins.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- The presenilins turned inside out: implications for their structures and functions.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- The ins and outs of presenilin 1 membrane topology.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Presenilin 1 suppresses the function of c-Jun homodimers via interaction with QM/Jif-1.The Journal of cell biology · 1999Article
- The biochemistry of Alzheimer's disease.Drugs & aging · 1999Review
- Presenilins: structural aspects and posttranslational events.Molecular neurobiology · 1999Review
- Specific intercellular binding of the beta-amyloid precursor protein to the presenilins induces intercellular signaling: its significance for Alzheimer's disease.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Additional evidence for an eight-transmembrane-domain topology for Caenorhabditis elegans and human presenilins.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- On the spurious endoproteolytic processing of the presenilin proteins in cultured cells and tissues.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Closing in on the amyloid cascade: recent insights into the cell biology of Alzheimer's disease.Molecular neurobiologyReview
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
To ascertain the membrane topography of the multi-transmembrane spanning presenilin proteins PS-1 and PS-2, anti-peptide antibodies were raised to several specific amino acid sequences in the two proteins, and, after their specificity was ascertained, the anti-peptide antibodies were used in immunofluorescent labeling of live PS-transfected, cultured DAMI cells, which are impermeable to the antibodies, as well as of their fixed and permeabilized counterparts. In such experiments, antibodies that specifically stain the intact live cells must label epitopes of the PS proteins that are on the exterior face of the plasma membrane whereas those antibodies that do not stain the live cells but do stain the fixed and permeabilized cells must label epitopes that face the cytoplasmic side of the membrane. The results obtained were entirely in accord with the predictions of the seven-transmembrane spanning topography (like that of rhodopsin and the beta-adrenergic receptor) and were totally inconsistent with the expectations for either the six- or eight-transmembrane topographies that have been proposed.
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