ArticleProceedings of the National Academy of Sciences of the United States of America1997
A ribozyme-mediated, gene "knockdown" strategy for the identification of gene function in zebrafish.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed, 42 citations in OpenAlex.
- Regulatory link mapping between organisms.BMC systems biology · 2011Article
- Development of lead hammerhead ribozyme candidates against human rod opsin mRNA for retinal degeneration therapy.Experimental eye research · 2009Article
- 15000 unique zebrafish EST clusters and their future use in microarray for profiling gene expression patterns during embryogenesis.Genome research · 2003Article
- Ribozyme-mediated resistance to rice dwarf virus and the transgene silencing in the progeny of transgenic rice plants.Transgenic research · 2000Article
- Diet-dependent hypercalciuria in transgenic mice with reduced CLC5 chloride channel expression.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Inhibition of luciferase expression by synthetic hammerhead ribozymes and their cellular uptake.Nucleic acids research · 1999Article
- Essential role of mitochondrially encoded large rRNA for germ-line formation in Drosophila embryos.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The zebrafish system offers many unique opportunities for the study of molecular biology. To date, only random mutagenesis, and not directed gene knockouts, have been demonstrated in this system. To more fully develop the potential of the zebrafish system, an approach to effectively inhibit the expression of any targeted gene in the developing zebrafish embryo has been developed. This approach uses a transient, cytoplasmic, T7 expression system, injected into the fertilized zebrafish egg to rapidly produce high levels of a ribozyme directed against the mRNA encoded by the targeted gene to inhibit its expression. In a demonstration of this strategy, expression of the recessive dominant zebrafish no tail gene was effectively inhibited by using this strategy to yield a phenotype identical to that resulting from a known defective mutation in this same gene. This, ribozyme-mediated, message deletion strategy may have use in determining the function of genetic coding sequences of unknown function.
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Registered trials
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