ArticleProceedings of the National Academy of Sciences of the United States of America1997
Methylation of the minimal promoter of an embryonic globin gene silences transcription in primary erythroid cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 88 citations in OpenAlex.
- Transposon Reactivation in the Germline May Be Useful for Both Transposons and Their Host Genomes.Cells · 2020Review
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- Molecular mechanisms of regulation and action of microRNA-199a in testicular germ cell tumor and glioblastomas.PloS one · 2013Article
- Article
- Prevention of transcriptional silencing by a replicator-binding complex consisting of SWI/SNF, MeCP1, and hnRNP C1/C2.Molecular and cellular biology · 2011Article
- MBD2 contributes to developmental silencing of the human ε-globin gene.Blood cells, molecules & diseases · 2011Article
- Advances in the understanding of haemoglobin switching.British journal of haematology · 2010Review
- Developmentally regulated extended domains of DNA hypomethylation encompass highly transcribed genes of the human beta-globin locus.Experimental hematology · 2009Article
- Epigenetics of beta-globin gene regulation.Mutation research · 2008Review
- Sp1/Sp3 and DNA-methylation contribute to basal transcriptional activation of human podoplanin in MG63 versus Saos-2 osteoblastic cells.BMC molecular biology · 2007Article
- Article
- Methylation mediated silencing of TMS1/ASC gene in prostate cancer.Molecular cancer · 2006Article
- Methyl binding domain protein 2 mediates gamma-globin gene silencing in adult human betaYAC transgenic mice.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Uroplakin Ib gene transcription in urothelial tumor cells is regulated by CpG methylation.Neoplasia (New York, N.Y.) · 2005Article
- Epigenetic regulation of 11 beta-hydroxysteroid dehydrogenase type 2 expression.The Journal of clinical investigation · 2004Article
- DNA methylation maintains allele-specific KIR gene expression in human natural killer cells.The Journal of experimental medicine · 2003Article
- Cytosine methylation of an Sp1 site contributes to organ-specific and cell-specific regulation of expression of the lung epithelial gene t1alpha.The Biochemical journal · 2000Article
- Human beta interferon scaffold attachment region inhibits de novo methylation and confers long-term, copy number-dependent expression to a retroviral vector.Journal of virology · 2000Article
- Dynamic analysis of proviral induction and De Novo methylation: implications for a histone deacetylase-independent, methylation density-dependent mechanism of transcriptional repression.Molecular and cellular biology · 2000Article
- Amelioration of retroviral vector silencing in locus control region beta-globin-transgenic mice and transduced F9 embryonic cells.Journal of virology · 1999Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Methylation of cytosines in the dinucleotide CpG has been shown to suppress transcription of a number of tissue-specific genes, yet the precise mechanism is not fully understood. The vertebrate globin genes were among the first examples in which an inverse correlation was shown between CpG methylation and transcription. We studied the methylation pattern of the 235-bp rho-globin gene promoter in genomic DNA from primary chicken erythroid cells using the sodium bisulfite conversion technique and found all CpGs in the promoter to be methylated in erythroid cells from adult chickens in which the rho-globin gene is silent but unmethylated in 5-day (primitive) embryonic red cells in which the gene is transcribed. To elucidate further the mechanism of methylation-induced silencing, an expression construct consisting of 235 bp of 5' promoter sequence of the rho-globin gene along with a strong 5' erythroid enhancer driving a chloramphenicol acetyltransferase reporter gene, rho-CAT, was transfected into primary avian erythroid cells derived from 5-day embryos. Methylation of just the 235-bp rho-globin gene promoter fragment at every CpG resulted in a 20- to 30-fold inhibition of transcription, and this effect was not overridden by the presence of potent erythroid-specific enhancers. The ability of the 235-bp rho-globin gene promoter to bind to a DNA Methyl Cytosine binding Protein Complex (MeCPC) was tested in electrophoretic mobility shift assays utilizing primary avian erythroid cell nuclear extract. The results were that fully methylated but not unmethylated 235-bp rho-globin gene promoter fragment could compete efficiently for MeCPC binding. These results are a direct demonstration that site-specific methylation of a globin gene promoter at the exact CpGs that are methylated in vivo can silence transcription in homologous primary erythroid cells. Further, these data implicate binding of MeCPC to the promoter in the mechanism of silencing.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.