ArticleProceedings of the National Academy of Sciences of the United States of America1997
The glyoxysomal and plastid molecular chaperones (70-kDa heat shock protein) of watermelon cotyledons are encoded by a single gene.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 88 citations in OpenAlex.
- Effects of Heat Stress on Plant-Nutrient Relations: An Update on Nutrient Uptake, Transport, and Assimilation.International journal of molecular sciences · 2023Review
- Plant peroxisome proteostasis-establishing, renovating, and dismantling the peroxisomal proteome.Essays in biochemistry · 2022Review
- BiFC Method Based on Intraorganellar Protein Crowding Detects Oleate-Dependent Peroxisomal Targeting ofInternational journal of molecular sciences · 2021Article
- Multi-layered molecular mechanisms of polypeptide holding, unfolding and disaggregation by HSP70/HSP110 chaperones.Frontiers in molecular biosciences · 2015Review
- Roles of Hsp70s in Stress Responses of Microorganisms, Plants, and Animals.BioMed research international · 2015Review
- Dual targeting of peroxisomal proteins.Frontiers in physiology · 2013Review
- Drought resistance in rice seedlings conferred by seed priming : role of the anti-oxidant defense mechanisms.Protoplasma · 2013Article
- Non-canonical peroxisome targeting signals: identification of novel PTS1 tripeptides and characterization of enhancer elements by computational permutation analysis.BMC plant biology · 2012Article
- Arabidopsis stromal 70-kDa heat shock proteins are essential for chloroplast development.Planta · 2010Article
- Arabidopsis glutathione reductase 1 is dually targeted to peroxisomes and the cytosol.Plant signaling & behavior · 2010Article
- Proteomic and selected metabolite analysis of grape berry tissues under well-watered and water-deficit stress conditions.Proteomics · 2009Article
- Peroxisome biogenesis and function.The arabidopsis book · 2009Article
- Identification and characterization of a stress-inducible and a constitutive small heat-shock protein targeted to the matrix of plant peroxisomes.Plant physiology · 2006Article
- Arabidopsis ACCELERATED CELL DEATH2 modulates programmed cell death.The Plant cell · 2006Article
- AraPerox. A database of putative Arabidopsis proteins from plant peroxisomes.Plant physiology · 2004Article
- Specification of the peroxisome targeting signals type 1 and type 2 of plant peroxisomes by bioinformatics analyses.Plant physiology · 2004Article
- Review
- Oxidative stress and acclimation mechanisms in plants.The arabidopsis book · 2002Article
- Dual targeting properties of the N-terminal signal sequence of Arabidopsis thaliana THI1 protein to mitochondria and chloroplasts.Plant molecular biology · 2001Article
- Cellular localization of isoprenoid biosynthetic enzymes in Marchantia polymorpha. Uncovering a new role of oil bodies.Plant physiology · 2000Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The monoclonal a-70-kDa heat shock protein (hsp70) antibody recognizes in crude extracts from watermelon (Citrullus vulgaris) cotyledons two hsps with molecular masses of 70 and 72 kDa. Immunocytochemistry on watermelon cotyledon tissue and on isolated glyoxysomes identified hsp70s in the matrix of glyoxysomes and plastids. Affinity purification and partial amino acid determination revealed the 70-kDa protein to share high sequence identity with cytosolic hsp70s from a number of plant species, while the 72 kDa protein was very similar to plastid hsp70s from pea and cucumber. A full-length cDNA clone encoding the 72-kDa hsp70 was isolated and identified two start methionines in frame within the N-terminal presequence leading either to an N-terminal extension of 67 amino acids or to a shorter one of 47 amino acids. The longer presequence was necessary and sufficient to target a reporter protein into watermelon proplastids in vitro. The shorter extension starting from the second methionine within the long version harbored a consensus peroxisomal targeting signal (RT-X5-KL) that directed in vivo a reporter protein into peroxisomes of the yeast Hansenula polymorpha. Peroxisomal targeting was however prevented, when the 67-residue presequence was fused to the reporter protein, indicating that the peroxisomal targeting signal 2 information is hidden in this context. We propose that the 72-kDa hsp70 is encoded by a single gene, but targeted alternatively into two organelles by the modulated use of its presequence.
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