ArticleProceedings of the National Academy of Sciences of the United States of America1997
Aldehyde dehydrogenase class 3 expression: identification of a cornea-preferred gene promoter in transgenic mice.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 56 citations in OpenAlex.
- Article
- Generation and characterization of Aldh3-Cre transgenic mice as a tool for conditional gene deletion in postnatal cornea.Scientific reports · 2020Article
- Corneal Expression of SLURP-1 by Age, Sex, Genetic Strain, and Ocular Surface Health.Investigative ophthalmology & visual science · 2015Article
- Ocular surface development and gene expression.Journal of ophthalmology · 2013Article
- Significant inhibition of corneal scarring in vivo with tissue-selective, targeted AAV5 decorin gene therapy.Investigative ophthalmology & visual science · 2011Article
- Overexpression of Pax6 in mouse cornea directly alters corneal epithelial cells: changes in immune function, vascularization, and differentiation.Investigative ophthalmology & visual science · 2011Article
- Efficacious and safe tissue-selective controlled gene therapy approaches for the cornea.PloS one · 2011Article
- Differential expression patterns and developmental roles of duplicated scinderin-like genes in zebrafish.Developmental dynamics : an official publication of the American Association of Anatomists · 2009Article
- Limbal stem cells in review.Journal of ophthalmic & vision research · 2009Article
- Investigation of the effects of PACAP on the composition of tear and endolymph proteins.Journal of molecular neuroscience : MN · 2008Article
- A role for chromosomal protein HMGN1 in corneal maturation.Differentiation; research in biological diversity · 2006Article
- Human aldehyde dehydrogenase 3A1 (ALDH3A1): biochemical characterization and immunohistochemical localization in the cornea.The Biochemical journal · 2003Article
- Structurally normal corneas in aldehyde dehydrogenase 3a1-deficient mice.Molecular and cellular biology · 2002Article
- Extrahepatic synthesis of plasminogen in the human cornea is up-regulated by interleukins-1alpha and -1beta.The Biochemical journal · 1999Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aldehyde dehydrogenase class 3 (ALDH3) constitutes 20-40% of the total water-soluble proteins in the mammalian cornea. Here, we show by Northern blot analysis that ALDH3 expression in the mouse is at least 500-fold higher in the cornea than in any other tissue examined, with very low levels of expression detected in the stomach, urinary bladder, ocular lens, and lung. Histochemical localization reveals that this exceptional level of expression in the mouse cornea occurs in the anterior epithelial cells and that little ALDH3 is present in the keratocytes or corneal endothelial cells. A 13-kbp mouse ALDH3 promoter fragment containing >12 kbp of the 5' flanking sequence, the 40-bp untranslated first exon, and 29 bp of intron 1 directed cat reporter gene expression to tissues that express the endogenous ALDH3 gene, except that transgene promoter activity was higher in the stomach and bladder than in the cornea. By contrast, when driven by a 4.4-kbp mouse ALDH3 promoter fragment [1,050-bp 5' flanking region, exon 1, intron 1 (3.4 kbp), and 7 bp of exon 2] expression of the cat reporter gene was confined to the corneal epithelial cells, except for very low levels in the liver, effectively reproducing the corneal expression pattern of the endogenous ALDH3 gene. These results indicate that tissue-specific expression of ALDH3 is determined by positive and negative elements in the 5' flanking region of the gene and suggests putative silencers located in intron 1. We demonstrate regulatory sequences capable of directing cornea-specific gene expression, affording the opportunity for genetic engineering in this transparent tissue.
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