ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1997
Myosin VIIA is required for aminoglycoside accumulation in cochlear hair cells.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed, 143 citations in OpenAlex.
- Extracellular vesicle-mediated gene editing for the treatment of nonsyndromic progressive hearing loss in adult mice.Science translational medicine · 2025Article
- Regeneration of Hair Cells from Endogenous Otic Progenitors in the Adult Mammalian Cochlea: Understanding Its Origins and Future Directions.International journal of molecular sciences · 2023Review
- Identifying targets to prevent aminoglycoside ototoxicity.Molecular and cellular neurosciences · 2022Review
- A natural knockout of the MYO7A gene leads to pre-weaning mortality in pigs.Animal genetics · 2021Article
- LGR5-Positive Supporting Cells Survive Ototoxic Trauma in the Adult Mouse Cochlea.Frontiers in molecular neuroscience · 2021Article
- Generation of inner ear hair cells by direct lineage conversion of primary somatic cells.eLife · 2020Article
- Systemic Fluorescent Gentamicin Enters Neonatal Mouse Hair Cells Predominantly Through Sensory Mechanoelectrical Transduction Channels.Journal of the Association for Research in Otolaryngology : JARO · 2020Article
- Necroptosis and Apoptosis Contribute to Cisplatin and Aminoglycoside Ototoxicity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- LMO7 deficiency reveals the significance of the cuticular plate for hearing function.Nature communications · 2019Article
- Water Waves to Sound Waves: Using Zebrafish to Explore Hair Cell Biology.Journal of the Association for Research in Otolaryngology : JARO · 2019Review
- Immunohistochemical localization of megalin and cubilin in the human inner ear.Brain research · 2018Article
- Kanamycin Damages Early Postnatal, but Not Adult Spiral Ganglion Neurons.Neurotoxicity research · 2017Article
- Potential Mechanisms Underlying Inflammation-Enhanced Aminoglycoside-Induced Cochleotoxicity.Frontiers in cellular neuroscience · 2017Review
- Endotoxemia-mediated inflammation potentiates aminoglycoside-induced ototoxicity.Science translational medicine · 2015Article
- Designer aminoglycosides prevent cochlear hair cell loss and hearing loss.The Journal of clinical investigation · 2015Article
- Mechanisms of sensorineural cell damage, death and survival in the cochlea.Frontiers in aging neuroscience · 2015Review
- myosin 7aa(-/-) mutant zebrafish show mild photoreceptor degeneration and reduced electroretinographic responses.Experimental eye research · 2014Article
- Functional and structural changes throughout the auditory system following congenital and early-onset deafness: implications for hearing restoration.Frontiers in systems neuroscience · 2013Review
- A novel role of cytosolic protein synthesis inhibition in aminoglycoside ototoxicity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Fish in a Dish: Drug Discovery for Hearing Habilitation.Drug discovery today. Disease models · 2013Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Myosin VIIA is expressed by sensory hair cells and has a primary structure predicting a role in membrane trafficking and turnover, processes that may underlie the susceptibility of hair cells to aminoglycoside antibiotics. [3H]Gentamicin accumulation and the effects of aminoglycosides were therefore examined in cochlear cultures of mice with different missense mutations in the myosin VIIA gene, Myo7a, to see whether myosin VIIA plays a role in aminoglycoside ototoxicity. Hair cells from homozygous mutant Myo7ash1 mice, with a mutation in a nonconserved region of the myosin VIIA head, respond rapidly to aminoglycoside treatment and accumulate high levels of gentamicin. Hair cells from homozygous mutant Myo7a6J mice, with a mutation at a highly conserved residue close to the ATP binding site of the myosin VIIA head, do not accumulate [3H]gentamicin and are protected from aminoglycoside ototoxicity. Hair cells from heterozygotes of both alleles accumulate [3H]gentamicin and respond to aminoglycosides. Although aminoglycoside uptake is thought to be via apical surface-associated endocytosis, coated pit numbers on the apical membrane of heterozygous and homozygous Myo7a6J hair cells are similar. Pulse-chase experiments with cationic ferritin confirm that the apical endocytotic pathway is functional in homozygous Myo7a6J hair cells. Transduction currents can be recorded from both heterozygous and homozygous Myo7a6J hair cells, suggesting it is unlikely that the drug enters via diffusion through the mechanotransducer channel. The results show that myosin VIIA is required for aminoglycoside accumulation in hair cells. Myosin VIIA may transport a putative aminoglycoside receptor to the hair cell surface, indirectly translocate it to sites of membrane retrieval, or retain it in the endocytotic pathway.
Indexed as
Identifiers
9391006PMC6573420W1921908229What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.