ArticleGenes & development1997
A novel cytosolic regulator, Pianissimo, is required for chemoattractant receptor and G protein-mediated activation of the 12 transmembrane domain adenylyl cyclase in Dictyostelium.
Article in Genes & development, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 128 citations in OpenAlex.
- The principles of directed cell migration.Nature reviews. Molecular cell biology · 2021Review
- Leep1 interacts with PIP3 and the Scar/WAVE complex to regulate cell migration and macropinocytosis.The Journal of cell biology · 2021Article
- The Dynamics of Aerotaxis in a Simple Eukaryotic Model.Frontiers in cell and developmental biology · 2021Article
- DPF is a cell-density sensing factor, with cell-autonomous and non-autonomous functions during Dictyostelium growth and development.BMC biology · 2019Article
- Proteomic and Transcriptomic Profiling Identifies Early Developmentally Regulated Proteins inCells · 2019Article
- Caffeine inhibits PI3K and mTORC2 in Dictyostelium and differentially affects multiple other cAMP chemoattractant signaling effectors.Molecular and cellular biochemistry · 2019Article
- Phosphorylated Rho-GDP directly activates mTORC2 kinase towards AKT through dimerization with Ras-GTP to regulate cell migration.Nature cell biology · 2019Article
- [MicroRNA-152 and microRNA-448 inhibit proliferation of colorectal cancer cellsNan fang yi ke da xue xue bao = Journal of Southern Medical University · 2019Article
- Dual TORCs driven and B56 orchestrated signaling network guides eukaryotic cell migration.BMB reports · 2017Article
- The small GTPases Ras and Rap1 bind to and control TORC2 activity.Scientific reports · 2016Article
- Signaling networks that regulate cell migration.Cold Spring Harbor perspectives in biology · 2015Review
- Genetic control of morphogenesis in Dictyostelium.Developmental biology · 2015Review
- A large-scale screen reveals genes that mediate electrotaxis in Dictyostelium discoideum.Science signaling · 2015Article
- Moving towards a paradigm: common mechanisms of chemotactic signaling in Dictyostelium and mammalian leukocytes.Cellular and molecular life sciences : CMLS · 2014Review
- The hybrid type polyketide synthase SteelyA is required for cAMP signalling in early Dictyostelium development.PloS one · 2014Article
- Rictor regulates cell migration by suppressing RhoGDI2.Oncogene · 2013Article
- A Gβγ effector, ElmoE, transduces GPCR signaling to the actin network during chemotaxis.Developmental cell · 2012Article
- Analysis of chemotaxis in Dictyostelium.Methods in molecular biology (Clifton, N.J.) · 2012Article
- TOR complex 2 (TORC2) in Dictyostelium suppresses phagocytic nutrient capture independently of TORC1-mediated nutrient sensing.Journal of cell science · 2012Article
- Direct biochemical measurements of signal relay during Dictyostelium development.The Journal of biological chemistry · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Genetic analysis was applied to identify novel genes involved in G protein-linked pathways controlling development. Using restriction enzyme-mediated integration (REMI), we have identified a new gene, Pianissimo (PiaA), involved in cAMP signaling in Dictyostelium discoideum. PiaA encodes a 130-kD cytosolic protein required for chemoattractant receptor and G protein-mediated activation of the 12 transmembrane domain adenylyl cyclase. In piaA- null mutants, neither chemoattractant stimulation of intact cells nor GTPgammaS treatment of lysates activates the enzyme; constitutive expression of PiaA reverses these defects. Cytosols of wild-type cells that contain Pia protein reconstitute the GTPgammaS stimulation of adenylyl cyclase activity in piaA- lysates, indicating that Pia is directly involved in the activation. Pia and CRAC, a previously identified cytosolic regulator, are both essential for activation of the enzyme as lysates of crac- piaA- double mutants require both proteins for reconstitution. Homologs of PiaA are found in Saccharomyces cerevisiae and Schizosaccaromyces pombe; disruption of the S. cerevisiae homolog results in lethality. We propose that homologs of Pia and similar modes of regulation of these ubiquitous G protein-linked pathways are likely to exist in higher eukaryotes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.