ArticleClinical and diagnostic laboratory immunology1997
Immunomagnetic selection of purified monocyte and lymphocyte populations from peripheral blood mononuclear cells following cryopreservation.
Article in Clinical and diagnostic laboratory immunology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT03269474 (Computational Drug Repurposing for All Epidermolysis Bullosa Simplex), which is not on this map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Computational Drug Repurposing for All Epidermolysis Bullosa Simplex (EBS) Cases
Who cites it
12 citing papers in PubMed.
- Profiling Tumor Infiltrating Immune Cells with CIBERSORT.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Immune Reconstitution but Persistent Activation After 48 Weeks of Antiretroviral Therapy in Youth With Pre-Therapy CD4 >350 in ATN 061.Journal of acquired immune deficiency syndromes (1999) · 2015Article
- The Center for HIV/AIDS Vaccine Immunology (CHAVI) multi-site quality assurance program for cryopreserved human peripheral blood mononuclear cells.Journal of immunological methods · 2014Article
- Viable mononuclear cell stability study for implementation in a proficiency testing program: impact of shipment conditions.Biopreservation and biobanking · 2014Article
- Standardized Serum-Free Cryomedia Maintain Peripheral Blood Mononuclear Cell Viability, Recovery, and Antigen-Specific T-Cell Response Compared to Fetal Calf Serum-Based Medium.Biopreservation and biobanking · 2011Article
- Antiretroviral therapy restores diversity in the T-cell receptor Vbeta repertoire of CD4 T-cell subpopulations among human immunodeficiency virus type 1-infected children and adolescents.Clinical and vaccine immunology : CVI · 2009Article
- Antiretroviral therapy corrects HIV-1-induced expansion of CD8+ CD45RA+ CD2-) CD11a(bright) activated T cells.The Journal of allergy and clinical immunology · 2008Article
- Quality assurance program for peripheral blood mononuclear cell cryopreservation.Clinical and vaccine immunology : CVI · 2007Article
- Complex determinants in human immunodeficiency virus type 1 envelope gp120 mediate CXCR4-dependent infection of macrophages.Journal of virology · 2005Article
- Human lymphocyte proliferation responses following primary immunization with rabies vaccine as neoantigen.Clinical and diagnostic laboratory immunology · 2001Article
- T-Cell receptor Vbeta repertoire CDR3 length diversity differs within CD45RA and CD45RO T-cell subsets in healthy and human immunodeficiency virus-infected children.Clinical and diagnostic laboratory immunology · 2000Article
- Expression of CCR5 increases during monocyte differentiation and directly mediates macrophage susceptibility to infection by human immunodeficiency virus type 1.Journal of virology · 1998Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Cryopreservation is a method commonly used to store human blood samples. We sought to determine if cryopreserved peripheral blood mononuclear cells (PBMC) could be separated effectively into distinct populations by using monoclonal antibodies and immunomagnetic microspheres. PBMC obtained from healthy blood donors and from human immunodeficiency virus-infected subjects were cryopreserved for as long as 18 months. Recovered cells were separated into CD14+ monocytes and CD4+ T-cell subsets by immunomagnetic selection. Flow cytometry analysis indicated >95% depletion of monocytes from PBMC following immunomagnetic selection with anti-CD14. A highly enriched population of CD4+ T cells was obtained from the CD14-depleted cell fraction by using an anti-CD4 monoclonal antibody and detachable immunomagnetic beads. The CD4+ T cells were subsequently separated into CD4+ CD45RO and CD4+ CD45RA fractions. Each fraction contained >90% enrichment for the respective subpopulation and <5% of the reciprocal subpopulation. No significant differences in cell surface expression of leukocyte markers, in efficiency of selection of PBMC subpopulations, or in mitogen-induced proliferation were detected in freshly isolated or cryopreserved cells. Efficient recovery of cryopreserved specimens means that targeted assays can be performed on selected, prospectively stored samples once clinical endpoints have been achieved.
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